ArticleIranian journal of pharmaceutical research : IJPR2011
Optimization of Chitinase Production by Bacillus pumilus Using Plackett-Burman Design and Response Surface Methodology.
Article in Iranian journal of pharmaceutical research : IJPR, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
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- Process optimisation for improved chitinase production from marine isolate Bacillus haynesii and bioethanol production with Saccharomyces cerevisiae.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Genomics, Proteomics, and Antifungal Activity of Chitinase from the Antarctic Marine BacteriumInternational journal of molecular sciences · 2024Article
- A Thermotolerant YeastJournal of fungi (Basel, Switzerland) · 2024Article
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- OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived FungusMarine drugs · 2021Article
- A Cold-Adapted Chitinase-Producing Bacterium from Antarctica and Its Potential in Biocontrol of Plant Pathogenic Fungi.Marine drugs · 2019Article
- Production of Vitamin DIranian journal of pharmaceutical research : IJPR · 2019Article
- Enhanced Production and Characterization of a Highly Stable Extracellular Protease from an Extreme Halophilic IsolateIranian journal of pharmaceutical research : IJPR · 2018Article
- Simultaneous Optimization of the Production of Organic Selenium and Cell Biomass inIranian journal of pharmaceutical research : IJPR · 2018Article
- Statistical design for formulation optimization of hydrocortisone butyrate-loaded PLGA nanoparticles.AAPS PharmSciTech · 2014Article
- Halotolerant Ability and α-Amylase Activity of Some Saltwater Fungal Isolates.Iranian journal of pharmaceutical research : IJPR · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A soil bacterium capable of degrading chitin on chitin agar plates was isolated and identified as Bacillus pumilus isolate U5 on the basis of 16S rDNA sequence analysis. In order to optimize culture conditions for chitinase production by this bacterium, a two step approach was employed. First, the effects of several medium components were studied using the Plackett-Burman design. Among various components tested, chitin and yeast extract showed positive effect on enzyme production while MgSO4 and FeSO4 had negative effect. However, the linear model proved to be insufficient for determining the optimum levels for these components due to a highly significant curvature effect. In the second step, Box-Behnken response surface methodology was used to determine the optimum values. It was noticed that a quadratic polynomial equation fitted he experimental data appropriately. The optimum concentrations for chitin, yeast extract, MgSO4 and FeSO4 were found to be 4.76, 0.439, 0.0055 and 0.019 g/L, respectively, with a predicted value of chitinase production of 97.67 U/100 mL. Using this statistically optimized medium, the practical chitinase production reached 96.1 U/100 mL.
Indexed as
Identifiers
24250411PMC3813072W2144985765What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.