ArticleTheScientificWorldJournal2013
The value of routine polymerase chain reaction analysis of intraocular fluid specimens in the diagnosis of infectious posterior uveitis.
Article in TheScientificWorldJournal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Epidemiological characterization of uveitis in the elderly population: a systematic review and meta-analysis.International ophthalmology · 2026Pooled it
- Amplification-Free Nanopore Sequencing for Herpesvirus DNA Detection in Intraocular Fluids.Ophthalmology science · 2026Article
- Diagnostic and therapeutic impact of PCR in uveitis: real-world data from intraocular fluid analysis of 45 uveitis patients in a tertiary referral center in Turkey.BMC ophthalmology · 2026Article
- Low-volume multiplex solid-phase PCR without DNA extraction for etiological diagnosis of infectious uveitis.Indian journal of ophthalmology · 2026Article
- The Use of CSF Multiplex PCR Panel in Patients with Viral Uveitis.Diagnostics (Basel, Switzerland) · 2026Article
- Polymerase chain reaction test for diagnosis of infectious uveitis.International journal of retina and vitreous · 2023Article
- Improved Detection of Herpesviruses from Diluted Vitreous Specimens Using Hydrogel Particles.Diagnostics (Basel, Switzerland) · 2022Article
- Article
- The Utility of Nonroutine Intraocular Fluid Polymerase Chain Reaction for Uveitis in Indonesia.Infection and drug resistance · 2022Article
- Usefulness of aqueous and vitreous humor analysis in infectious uveitis.Clinics (Sao Paulo, Brazil) · 2020Article
- Real-time polymerase chain reaction in recurrent cytomegalovirus anterior uveitis.American journal of ophthalmology case reports · 2019Article
- Tuberculosis and other causes of uveitis in Indonesia.Eye (London, England) · 2018Article
- Aqueous humor polymerase chain reaction in uveitis - utility and safety.BMC ophthalmology · 2016Article
- Polymerase chain reaction to search for Herpes viruses in uveitic and healthy eyes: a South African perspective.African health sciences · 2015Article
- Long-term follow-up of herpetic non-necrotizing retinopathy with occlusive retinal vasculitis and neovascularization.Journal of ophthalmic inflammation and infection · 2015Article
- Viral posterior uveitis.Survey of ophthalmologyReview
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo assess the value of routine polymerase chain reaction (PCR) analysis on intraocular fluid from patients presenting with a first episode of suspected active infectious posterior uveitis in a population with a high prevalence of human immunodeficiency virus infection.
designRetrospective, interventional case series. Participants. 159 consecutive patients presenting at a tertiary care hospital over a five-year period.
methodsPCR analysis was performed for cytomegalovirus, varicella zoster virus, herpes simplex virus types 1 and 2, Toxoplasma gondii, and Mycobacterium tuberculosis.
resultsPCR analysis confirmed the initial clinical diagnosis in 55 patients (35%) and altered the initial clinical diagnosis in 36 patients (23%). The clinical diagnosis prior to PCR testing was nonspecific (uncertain) in 51 patients (32%), with PCR providing a definitive final diagnosis in 20 of these patients (39%); necrotizing herpetic retinopathy and ocular toxoplasmosis were particularly difficult to diagnose correctly without the use of PCR analysis.
conclusionThe clinical phenotype alone was unreliable in diagnosing the underlying infectious cause in a quarter of patients in this study. Since the outcome of incorrectly treated infective uveitis can be blinding, PCR analysis of ocular fluids is recommended early in the disease even in resource poor settings.
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