ArticleThe Plant cell2013
A BAR-domain protein SH3P2, which binds to phosphatidylinositol 3-phosphate and ATG8, regulates autophagosome formation in Arabidopsis.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 130 papers, 1 of them a synthesis that pooled it.
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Who cites it
130 citing papers in PubMed, 1 synthesis or guideline pooled it, 243 citations in OpenAlex.
- Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)Autophagy · 2021Guideline
- Beyond parallel pathways: molecular integration and functional crosstalk between autophagy and endocytosis in plants.Protoplasma · 2026Review
- Structural Features of Root Cells ofInternational journal of molecular sciences · 2026Article
- OsVPS34-generated PI3P recruits GPA5/Rab5a to regulate post-Golgi glutelin trafficking in rice endosperm.Plant physiology · 2026Article
- A dual phospholipase system instructs membrane hydrolysis during the final stages of plant autophagy.Nature communications · 2026Article
- Biomolecular condensation of ERC1 recruits ATG8 and NBR1 to drive autophagosome formation for plant heat tolerance.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Vacuolar sorting receptors coordinate lytic vacuolar and autophagic transport for plant effector-triggered immunity.Nature plants · 2025Article
- Plant-specific mitochondrial fission machinery connects mitochondrial dynamics and mitophagosome formation for piecemeal mitophagy.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Autophagy deficiency confers freezing tolerance in Arabidopsis thaliana.BMC plant biology · 2025Article
- ATG8ylation-mediated tonoplast invagination mitigates vacuole damage.Nature communications · 2025Article
- The N-degron pathway governs autophagy to promote thermotolerance in Arabidopsis.Nature communications · 2025Article
- Myotubularin 2 interacts with SEC23A and negatively regulates autophagy at ER exit sites in Arabidopsis.Autophagy · 2025Article
- The MON1-CCZ1 complex plays dual roles in autophagic degradation and vacuolar protein transport in rice.Journal of integrative plant biology · 2025Article
- 14-3-3 proteins inhibit autophagy by regulating SINAT-mediated proteolysis of ATG6 in Arabidopsis.BMC plant biology · 2024Article
- The Role of Female and Male Genes in Regulating Pollen Tube Guidance in Flowering Plants.Genes · 2024Review
- Vacuolar degradation of plant organelles.The Plant cell · 2024Review
- Autophagosome biogenesis and organelle homeostasis in plant cells.The Plant cell · 2024Review
- Clathrin light chains negatively regulate plant immunity by hijacking the autophagy pathway.Plant communications · 2024Article
- Full-length transcriptome sequencing of Arabidopsis plants provided new insights into the autophagic regulation of photosynthesis.Scientific reports · 2024Article
- A century journey of organelles research in the plant endomembrane system.The Plant cell · 2024Review
70 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autophagy is a well-defined catabolic mechanism whereby cytoplasmic materials are engulfed into a structure termed the autophagosome. In plants, little is known about the underlying mechanism of autophagosome formation. In this study, we report that SH3 DOMAIN-CONTAINING PROTEIN2 (SH3P2), a Bin-Amphiphysin-Rvs domain-containing protein, translocates to the phagophore assembly site/preautophagosome structure (PAS) upon autophagy induction and actively participates in the membrane deformation process. Using the SH3P2-green fluorescent protein fusion as a reporter, we found that the PAS develops from a cup-shaped isolation membranes or endoplasmic reticulum-derived omegasome-like structures. Using an inducible RNA interference (RNAi) approach, we show that RNAi knockdown of SH3P2 is developmentally lethal and significantly suppresses autophagosome formation. An in vitro membrane/lipid binding assay demonstrates that SH3P2 is a membrane-associated protein that binds to phosphatidylinositol 3-phosphate. SH3P2 may facilitate membrane expansion or maturation in coordination with the phosphatidylinositol 3-kinase (PI3K) complex during autophagy, as SH3P2 promotes PI3K foci formation, while PI3K inhibitor treatment inhibits SH3P2 from translocating to autophagosomes. Further interaction analysis shows that SH3P2 associates with the PI3K complex and interacts with ATG8s in Arabidopsis thaliana, whereby SH3P2 may mediate autophagy. Thus, our study has identified SH3P2 as a novel regulator of autophagy and provided a conserved model for autophagosome biogenesis in Arabidopsis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.