ArticleThe Plant cell2013
Subchromoplast sequestration of carotenoids affects regulatory mechanisms in tomato lines expressing different carotenoid gene combinations.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 127 citations in OpenAlex.
- Metabolic cues are the main drivers for the differentiation of chloroplasts into chromoplasts and other plastid types.Journal of experimental botany · 2026Review
- Decoding Isoprenoid Transcript-Metabolite Interactions in Carotenoid Tomato Fruit Mutants Uncovers Novel Metabolic Cross-Links.International journal of molecular sciences · 2026Article
- Carotenoid aggregates negatively impact chlorophyll levels and disrupt chloroplast development in peaches.Molecular horticulture · 2026Article
- The carotenoids metabolism during tomato fruit ripening: insights into multi-level regulatory network.BMC plant biology · 2026Review
- The FIBRILLIN multigene family in tomato, their roles in plastoglobuli structure and metabolism.The Plant journal : for cell and molecular biology · 2025Article
- Identification of a highly efficient chloroplast-targeting peptide for plastid engineering.PLoS biology · 2024Article
- Activation of the native PHYTOENE SYNTHASE 1 promoter by modifying near-miss cis-acting elements induces carotenoid biosynthesis in embryogenic rice callus.Plant cell reports · 2024Article
- Ketocarotenoid production in tomato triggers metabolic reprogramming and cellular adaptation: The quest for homeostasis.Plant biotechnology journal · 2024Article
- Carotenoid composition and sequestration in cassava (Manihot esculentum Crantz) roots.PloS one · 2024Article
- Light quality regulates plant biomass and fruit quality through a photoreceptor-dependent HY5-LHC/CYCB module in tomato.Horticulture research · 2023Article
- Effect of Low Light on Photosynthetic Performance of Tomato Plants-Ailsa Craig and Carotenoid MutantPlants (Basel, Switzerland) · 2023Article
- Biosynthesis of Chlorophyll and Other Isoprenoids in the Plastid of Red Grape Berry Skins.Journal of agricultural and food chemistry · 2023Article
- Gardenia carotenoid cleavage dioxygenase 4a is an efficient tool for biotechnological production of crocins in green and non-green plant tissues.Plant biotechnology journal · 2022Article
- Datasets from harmonised metabolic phenotyping of root, tuber and banana crop.Data in brief · 2022Article
- Comparative metabolomic and transcriptomic analysis reveals a coexpression network of the carotenoid metabolism pathway in the panicle of Setaria italica.BMC plant biology · 2022Article
- Riboswitch-mediated inducible expression of an astaxanthin biosynthetic operon in plastids.Plant physiology · 2022Article
- Going red but not mad: efficient astaxanthin production in tobacco without yield penalty.Plant physiology · 2022Article
- A comprehensive analysis of carotenoids metabolism in two red-fleshed mutants of Navel and Valencia sweet oranges (Frontiers in plant science · 2022Article
- Article
- Differential hydroxylation efficiency of the two non-heme carotene hydroxylases: DcBCH1, rather than DcBCH2, plays a major role in carrot taproot.Horticulture research · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic engineering of the carotenoid pathway in recent years has successfully enhanced the carotenoid contents of crop plants. It is now clear that only increasing biosynthesis is restrictive, as mechanisms to sequestrate these increased levels in the cell or organelle should be exploited. In this study, biosynthetic pathway genes were overexpressed in tomato (Solanum lycopersicum) lines and the effects on carotenoid formation and sequestration revealed. The bacterial Crt carotenogenic genes, independently or in combination, and their zygosity affect the production of carotenoids. Transcription of the pathway genes was perturbed, whereby the tissue specificity of transcripts was altered. Changes in the steady state levels of metabolites in unrelated sectors of metabolism were found. Of particular interest was a concurrent increase of the plastid-localized lipid monogalactodiacylglycerol with carotenoids along with membranous subcellular structures. The carotenoids, proteins, and lipids in the subchromoplast fractions of the transgenic tomato fruit with increased carotenoid content suggest that cellular structures can adapt to facilitate the sequestration of the newly formed products. Moreover, phytoene, the precursor of the pathway, was identified in the plastoglobule, whereas the biosynthetic enzymes were in the membranes. The implications of these findings with respect to novel pathway regulation mechanisms are discussed.
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