ArticleProceedings of the National Academy of Sciences of the United States of America2013
Pyrethroid activity-based probes for profiling cytochrome P450 activities associated with insecticide interactions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
- Predictive chemoproteomics and functional validation reveal Coeae6g-mediated insecticide cross-resistance in the malaria vector Anopheles gambiae.Nature communications · 2025Article
- Discovery of active mouse, plant and fungal cytochrome P450s in endogenous proteomes and upon expression in planta.Scientific reports · 2024Article
- Improved deconvolution of natural products' protein targets using diagnostic ions from chemical proteomics linkers.Beilstein journal of organic chemistry · 2024Review
- Activity-Based Protein Profiling (ABPP) of Oxidoreductases.Chembiochem : a European journal of chemical biology · 2021Review
- New insecticide screening platforms indicate that Mitochondrial Complex I inhibitors are susceptible to cross-resistance by mosquito P450s that metabolise pyrethroids.Scientific reports · 2020Article
- The endothelin receptor antagonist macitentan for the treatment of pulmonary arterial hypertension: A cross-species comparison of its cytochrome P450 induction pattern.Pharmacology research & perspectives · 2020Article
- High-Fat Diets Alter the Modulatory Effects of Xenobiotics on Cytochrome P450 Activities.Chemical research in toxicology · 2018Article
- Contemporary status of insecticide resistance in the major Aedes vectors of arboviruses infecting humans.PLoS neglected tropical diseases · 2017Review
- Hepatic Cytochrome P450 Activity, Abundance, and Expression Throughout Human Development.Drug metabolism and disposition: the biological fate of chemicals · 2016Article
- Chemical proteomics approaches for identifying the cellular targets of natural products.Natural product reports · 2016Review
- Artemisinin activity-based probes identify multiple molecular targets within the asexual stage of the malaria parasites Plasmodium falciparum 3D7.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- RNA-seq analyses of changes in the Anopheles gambiae transcriptome associated with resistance to pyrethroids in Kenya: identification of candidate-resistance genes and candidate-resistance SNPs.Parasites & vectors · 2015Article
- Expression analysis of two P450 monooxygenase genes of the tobacco cutworm moth (Spodoptera litura) at different developmental stages and in response to plant allelochemicals.Journal of chemical ecology · 2015Article
- Fit for purpose: do we have the right tools to sustain NTD elimination?BMC proceedings · 2015Article
- Dissecting the organ specificity of insecticide resistance candidate genes in Anopheles gambiae: known and novel candidate genes.BMC genomics · 2014Article
- Identification and characterisation of Aedes aegypti aldehyde dehydrogenases involved in pyrethroid metabolism.PloS one · 2014Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
Abstract
Pyrethroid insecticides are used to control diseases spread by arthropods. We have developed a suite of pyrethroid mimetic activity-based probes (PyABPs) to selectively label and identify P450s associated with pyrethroid metabolism. The probes were screened against pyrethroid-metabolizing and nonmetabolizing mosquito P450s, as well as rodent microsomes, to measure labeling specificity, plus cytochrome P450 oxidoreductase and b5 knockout mouse livers to validate P450 activation and establish the role for b5 in probe activation. Using PyABPs, we were able to profile active enzymes in rat liver microsomes and identify pyrethroid-metabolizing enzymes in the target tissue. These included P450s as well as related detoxification enzymes, notably UDP-glucuronosyltransferases, suggesting a network of associated pyrethroid-metabolizing enzymes, or "pyrethrome." Considering the central role P450s play in metabolizing insecticides, we anticipate that PyABPs will aid in the identification and profiling of P450s associated with insecticide pharmacology in a wide range of species, improving understanding of P450-insecticide interactions and aiding the development of unique tools for disease control.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.