ArticleProceedings of the National Academy of Sciences of the United States of America2013
Three proteins mediate import of transit sequence-less precursors into the inner envelope of chloroplasts in Arabidopsis thaliana.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 26 citations in OpenAlex.
- Genome-Wide Identification and Expression Analysis of the TIM Gene Family in Sea-Island Cotton (Current issues in molecular biology · 2026Article
- Advances and opportunities for computational interrogation of plant proteins.The Plant journal : for cell and molecular biology · 2026Review
- Understanding protein import in diverse non-green plastids.Frontiers in genetics · 2023Review
- New Insights into the Chloroplast Outer Membrane Proteome and Associated Targeting Pathways.International journal of molecular sciences · 2022Review
- The Eucalyptus grandis chloroplast proteome: Seasonal variations in leaf development.PloS one · 2022Article
- PRAT Proteins Operate in Organellar Protein Import and Export inPlants (Basel, Switzerland) · 2021Article
- Tim17 Updates: A Comprehensive Review of an Ancient Mitochondrial Protein Translocator.Biomolecules · 2020Review
- A Protochlorophyllide (Pchlide)Frontiers in plant science · 2019Article
- Ion and metabolite transport in the chloroplast of algae: lessons from land plants.Cellular and molecular life sciences : CMLS · 2018Review
- Loss of a highly conserved sterile alpha motif domain gene (Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Plant-Specific Preprotein and Amino Acid Transporter Proteins Are Required for tRNA Import into Mitochondria.Plant physiology · 2016Article
- Evolution of the Tim17 protein family.Biology direct · 2016Article
- Physiological implications of arginine metabolism in plants.Frontiers in plant science · 2015Review
- New functions of the chloroplast Preprotein and Amino acid Transporter (PRAT) family members in protein import.Plant signaling & behavior · 2014Review
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A family of 17 putative preprotein and amino acid transporters designated PRAT has been identified in Arabidopsis thaliana, comprising PRAT proteins in mitochondria and chloroplasts. Although some PRAT proteins, such as the translocon of the mitochondrial inner membrane (TIM) proteins TIM22 and TIM23, play decisive roles for the translocation and import of mitochondrial inner membrane proteins, little is known about the role of the different PRAT members in chloroplasts. Here we report the identification of three distinct PRAT proteins as part of a unique protein import site. One of the identified PRAT proteins is identical with a previously characterized hypothetical protein (HP) of 20 kDa designated HP20 of the outer plastid envelope membrane. The second PRAT component is represented by HP30, and the third is identical to HP30-2, a close relative of HP30. Both HP30 and HP30-2 are inner plastid envelope membrane proteins of chloroplasts. Using biochemical, cell biological, and genetic approaches we demonstrate that all three PRAT proteins cooperate during import of transit sequence-less proteins, such as the quinone oxidoreductase homolog ceQORH used as model, into the inner chloroplast envelope membrane. Our data are reminiscent of findings reported for the TIM22 translocase, which is involved in the import of carrier proteins and other, hydrophobic membrane proteins lacking cleavable transit sequences into the inner mitochondrial membrane. Together our results establish the PRAT family as a widely used system of protein translocases in different membranes of endosymbiotic origin.
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