ArticleProceedings of the National Academy of Sciences of the United States of America2013
Phosphorylation of the Rab exchange factor Sec2p directs a switch in regulatory binding partners.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- Osh-dependent and -independent Regulation of PI4P Levels During Polarized Growth ofMolecular biology of the cell · 2023Article
- Exploring the consequences of redirecting an exocytic Rab onto endocytic vesicles.Molecular biology of the cell · 2023Article
- Aspergillus Niger thermostable Cytosine deaminase-dextran conjugates with enhanced structure stability, proteolytic resistance, and Antiproliferative activity.BMC microbiology · 2023Article
- High-resolution secretory timeline from vesicle formation at the Golgi to fusion at the plasma membrane ineLife · 2022Article
- Who's in control? Principles of Rab GTPase activation in endolysosomal membrane trafficking and beyond.The Journal of cell biology · 2021Review
- Direct trafficking pathways from the Golgi apparatus to the plasma membrane.Seminars in cell & developmental biology · 2020Review
- Meiosis-specific localization of the exocytic Rab Ypt2 in fission yeast.Small GTPases · 2020Article
- Restoring the Taxol biosynthetic machinery of Aspergillus terreus by Podocarpus gracilior Pilger microbiome, with retrieving the ribosome biogenesis proteins of WD40 superfamily.Scientific reports · 2019Article
- Autoactivation of small GTPases by the GEF-effector positive feedback modules.F1000Research · 2019Review
- Efficient protein production by yeast requires global tuning of metabolism.Nature communications · 2017Article
- Moderate Expression ofApplied and environmental microbiology · 2017Article
- Regulation of membrane traffic by Rab GEF and GAP cascades.Small GTPases · 2016Review
- The casein kinases Yck1p and Yck2p act in the secretory pathway, in part, by regulating the Rab exchange factor Sec2p.Molecular biology of the cell · 2016Article
- Septin-Dependent Assembly of the Exocyst Is Essential for Plant Infection by Magnaporthe oryzae.The Plant cell · 2015Article
- Novel topography of the Rab11-effector interaction network within a ciliary membrane targeting complex.Small GTPases · 2015Review
- Tracking individual secretory vesicles during exocytosis reveals an ordered and regulated process.The Journal of cell biology · 2015Article
- Yeast vacuolar HOPS, regulated by its kinase, exploits affinities for acidic lipids and Rab:GTP for membrane binding and to catalyze tethering and fusion.Molecular biology of the cell · 2015Article
- Activation of Rab8 guanine nucleotide exchange factor Rabin8 by ERK1/2 in response to EGF signaling.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Genome-Wide Transcription Study of Cryptococcus neoformans H99 Clinical Strain versus Environmental Strains.PloS one · 2015Article
- Transcriptional and Proteomic Profiling of Aspergillus flavipes in Response to Sulfur Starvation.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Sec2p is a guanine nucleotide exchange factor that promotes exocytosis by activating the Rab GTPase Sec4p. Sec2p is highly phosphorylated, and we have explored the role of phosphorylation in the regulation of its function. We have identified three phosphosites and demonstrate that phosphorylation regulates the interaction of Sec2p with its binding partners Ypt32p, Sec15p, and phosphatidyl-inositol-4-phosphate. In its nonphosphorylated form, Sec2p binds preferentially to the upstream Rab, Ypt32p-GTP, thus forming a Rab guanine nucleotide exchange factor cascade that leads to the activation of the downstream Rab, Sec4p. The nonphosphorylated form of Sec2p also binds to the Golgi-associated phosphatidyl-inositol-4-phosphate, which works in concert with Ypt32p-GTP to recruit Sec2p to Golgi-derived secretory vesicles. In contrast, the phosphorylated form of Sec2p binds preferentially to Sec15p, a downstream effector of Sec4p and a component of the exocyst tethering complex, thus forming a positive-feedback loop that prepares the secretory vesicle for fusion with the plasma membrane. Our results suggest that the phosphorylation state of Sec2p can direct a switch in its regulatory binding partners that facilitates maturation of the secretory vesicle and helps to promote the directionality of vesicular transport.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.