ArticleMedical principles and practice : international journal of the Kuwait University, Health Science Centre2014
The protective effect of hesperidin on methotrexate-induced intestinal epithelial damage in rats: an experimental study.
Article in Medical principles and practice : international journal of the Kuwait University, Health Science Centre, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- Molecular mechanisms underlying methotrexate-induced intestinal injury and protective strategies.Naunyn-Schmiedeberg's archives of pharmacology · 2024Review
- Pseudomonas aeruginosa Keratitis in Rats: Study of the Effect of Topical 5% Hesperidin Practice on Healing.The Eurasian journal of medicine · 2023Article
- Protective effects of hesperidin in cyclophosphamide-induced parotid toxicity in rats.Scientific reports · 2023Article
- Protective Effect of Bojungikki-Tang against Radiation-Induced Intestinal Injury in Mice: Experimental Verification and Compound-Target Prediction.Evidence-based complementary and alternative medicine : eCAM · 2023Article
- Hesperidin Alleviates Methotrexate-Induced Memory Deficits via Hippocampal Neurogenesis in Adult Rats.Nutrients · 2019Article
- Protective Effects of 2-Amino-5,6-dihydro-4International journal of molecular sciences · 2018Article
- The Impact of Resveratrol on Oxidative Stress Induced by Methotrexate in Rat Ileum Tissue: Evaluation of Biochemical and Histopathological Features and Analysis of Gene Expression.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2016Article
- Protective effects of carvacrol and pomegranate against methotrexate-induced intestinal damage in rats.International journal of clinical and experimental medicine · 2015Article
- Protective effect of resveratrol against methotrexate-induced oxidative stress in the small intestinal tissues of rats.International journal of clinical and experimental medicine · 2015Article
- Topical hesperidin prevents glucocorticoid-induced abnormalities in epidermal barrier function in murine skin.Experimental dermatology · 2014Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe purpose of this experimental study was to evaluate the efficacy of hesperidin (HES) in protecting against methotrexate (MTX)-induced intestinal damage using histopathological and immunohistochemical techniques. MATERIALS AND
methodsSeventy-eight male Wistar albino rats were divided into 4 groups that received (a) saline only (control group), n = 19; (b) HES only, n = 19; (c) MTX only, n = 19, and (d) MTX plus HES, n = 21. On the first day of the study, a single dose of MTX (20 mg/kg) was administered intraperitoneally to group 3 and 4 rats. The HES (200 mg/kg) was administered by gavage for 5 days. For the MTX plus HES group, HES (200 mg/kg) was administered by gavage for 5 days after MTX treatment. Rats were sacrificed on the 2nd, 4th and 6th day of the study. Tissue samples from the jejunum were taken for histopathological and immunohistochemical analysis.
resultsOn the 4th day, crypt injury in the MTX plus HES group (1.00 ± 0.00) was less than that in the MTX group (2.00 ± 0.89; p < 0.05). The small intestinal damage score was lower in the MTX plus HES group (6.33 ± 0.82) as compared to the MTX group (8.00 ± 2.37). Inducible nitric oxide synthase and interleukin-8 levels were lower in the MTX plus HES group (65 and 25%, respectively) as compared to the corresponding values of the MTX group (80 and 52.5%, respectively). On the 6th day, the Ki-67 proliferation index in the MTX group (45%) was lower than that in the MTX plus HES group (76.67%) and the control group (p < 0.05). The small intestinal damage score was high in the HES group on the 4th day due to increased cellular infiltration. On the 6th day, the Ki-67 proliferation index rose in parallel with the decrease in cellular infiltration and therefore histopathological scoring. The proliferation-enhancing effect of HES also appeared in healthy rats.
conclusionHES seemed to have a protective effect against MTX-induced intestinal injury.
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