ArticleAntimicrobial agents and chemotherapy2014
Detection and molecular characterization of Escherichia coli CTX-M-15 and Klebsiella pneumoniae SHV-12 β-lactamases from bovine mastitis isolates in the United Kingdom.
Article in Antimicrobial agents and chemotherapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 93 citations in OpenAlex.
- Prevalence and risk factors ofFrontiers in veterinary science · 2023Pooled it
- Prevalence and Antimicrobial Resistance of Klebsiella pneumoniae Isolated from Subclinical Mastitis in Selected Pure Dairy Cattle Breeds in Pakistan.Current microbiology · 2025Article
- Antimicrobial resistance and molecular epidemiology of Klebsiella pneumoniae isolated from bovine mastitis in seven provinces in China.BMC microbiology · 2025Article
- Antimicrobial resistance profiles and molecular epidemiology ofEpidemiology and infection · 2025Article
- Extended-Spectrum Beta-Lactamase-ProducingAntibiotics (Basel, Switzerland) · 2024Review
- Antibiogram ofVeterinary medicine (Auckland, N.Z.) · 2024Article
- Bovine mastitis, a worldwide impact disease: Prevalence, antimicrobial resistance, and viable alternative approaches.Veterinary and animal science · 2023Review
- Molecular epidemiology and pathogenomics of extended-spectrum beta-lactamase producing-Frontiers in microbiology · 2023Article
- Article
- Subclinical mastitis in dairy cows in south-Asian countries: a review of risk factors and etiology to prioritize control measures.Veterinary research communications · 2022Review
- Lineages, Virulence Gene Associated and Integrons among Extended Spectrum β-Lactamase (ESBL) and CMY-2 ProducingPathogens (Basel, Switzerland) · 2022Article
- Phenotyping and genotyping studies on extended-spectrum β-lactamase-producingVeterinary world · 2022Article
- Epidemiology, Environmental Risks, Virulence, and Resistance Determinants ofFrontiers in microbiology · 2022Article
- Article
- Molecular characterization of KPC-2-producing Klebsiella pneumoniae ST258 isolated from bovine mastitis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Review on the occurrence of theHeliyon · 2021Review
- Prevalence and antimicrobial resistance ofJournal of veterinary science · 2021Article
- Characterization of Enterococci- and ESBL-ProducingPathogens (Basel, Switzerland) · 2021Article
- Prevalence, Antimicrobial Susceptibility, and Molecular Characterization ofFrontiers in microbiology · 2021Article
- Molecular characterization of extended-spectrum β-lactamase-producingVeterinary world · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent reports raised concerns about the role that farm stock may play in the dissemination of extended-spectrum β-lactamase (ESBL)-producing bacteria. This study characterized the ESBLs in two Escherichia coli and three Klebsiella pneumoniae subsp. pneumoniae isolates from cases of clinical bovine mastitis in the United Kingdom. Bacterial culture and sensitivity testing of bovine mastitic milk samples identified Gram-negative cefpodoxime-resistant isolates, which were assessed for their ESBL phenotypes. Conjugation experiments and PCR-based replicon typing (PBRT) were used for characterization of transferable plasmids. E. coli isolates belonged to sequence type 88 (ST88; determined by multilocus sequence typing) and carried blaCTX-M-15 and blaTEM-1, while K. pneumoniae subsp. pneumoniae isolates carried blaSHV-12 and blaTEM-1. Conjugation experiments demonstrated that blaCTX-M-15 and blaTEM-1 were carried on a conjugative plasmid in E. coli, and PBRT identified this to be an IncI1 plasmid. The resistance genes were nontransferable in K. pneumoniae subsp. pneumoniae isolates. Moreover, in the E. coli isolates, an association of ISEcp1 and IS26 with blaCTX-M-15 was found where the IS26 element was inserted upstream of both ISEcp1 and the blaCTX-M promoter, a genetic arrangement highly similar to that described in some United Kingdom human isolates. We report the first cases in Europe of bovine mastitis due to E. coli CTX-M-15 and also of bovine mastitis due to K. pneumoniae subsp. pneumoniae SHV-12 β-lactamases in the United Kingdom. We also describe the genetic environment of blaCTX-M-15 and highlight the role that IncI1 plasmids may play in the spread and dissemination of ESBL genes, which have been described in both human and cattle isolates.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.