Evidence map›Paper›PMID 24246745›Full record

ArticleExperimental hematology2014

Changes in the frequencies of human hematopoietic stem and progenitor cells with age and site.

Tracy L Farrell, Timothy R McGuire, Laura D Bilek, Susan K Brusnahan, John D Jackson, Judy T Lane, Kevin L Garvin, Barbara J O'Kane, Ann M Berger, Sonal R Tuljapurkar and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental hematology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Cell Sources for Human In vitro Bone Models.Current osteoporosis reports · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Tracy L FarrellDepartment of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USA.
Timothy R McGuirePharmacy Practice, University of Nebraska Medical Center, Omaha, NE, USA.
Laura D BilekSchool of Allied Health Professions, University of Nebraska Medical Center, Omaha, NE, USA.
Susan K BrusnahanPharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE, USA.
John D JacksonInstitute for Regenerative Medicine, Wake Forest University, Winston-Salem, NC, USA.
Judy T LanePulmonary, Critical Care and Sleep Medicine, University of Nebraska Medical Center, Omaha, NE, USA.
Kevin L GarvinOrthopaedic Surgery and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA.
Barbara J O'KaneSchool of Dentistry, Creighton University, Omaha, NE, USA.
Ann M BergerAdult Health and Illness, College of Nursing, University of Nebraska Medical Center, Omaha, NE, USA.
Sonal R TuljapurkarDepartment of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USA.
M Anne KessingerInternal Medicine Oncology/Hematology, University of Nebraska Medical Center, Omaha, NE, USA.
John Graham SharpDepartment of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USA. Electronic address: jsharp@unmc.edu.
University of Nebraska Medical Center · USCreighton University · USWake Forest University · US

Funding

Stem Cell Quality Assays: Correlation with Aging/HealthR01AG024912 · NIA · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI SHARP, JOHN GRAHAM · 2004 to 2010
$1.5M
NIA NIH HHS R01 AG024912
6 · The paper itself

Abstract

This study enumerated CD45(hi)/CD34(+) and CD45(hi)/CD133(+) human hematopoietic stem cells (HSCs) and progenitor granulocyte-macrophage colony forming cells (GM-CFCs) in blood and trochanteric and femoral bone marrow in 233 individuals. Stem cell frequencies were determined with multiparameter flow cytometry and using an internal control to determine the intrinsic variance of the assays. Progenitor cell frequency was determined using a standard colony assay technique. The frequency of outliers from undetermined methodological causes was highest for blood, but less than 5% for all values. The frequency of CD45(hi)/CD133(+) cells correlated highly with the frequency of CD45(hi)/CD34(+) cells in trochanteric and femoral bone marrow. The frequency of these HSC populations in trochanteric and femoral bone marrow rose significantly with age. In contrast, there was no significant trend of either of these cell populations with age in the blood. Trochanteric marrow progenitor GM-CFCs showed no significant trends with age, but femoral marrow GM-CFCs trended downward with age, potentially because of the reported conversion of red marrow at this site to fat with age. Hematopoietic stem and progenitor cells exhibited changes in frequencies with age that differed between blood and bone marrow. We previously reported that side population (SP) multipotential HSC, which includes the precursors of CD45(hi)/CD133(+) and CD45(hi)/CD34(+), decline with age. Potentially the increases in stem cell frequencies in the intermediate compartment between SP and GM progenitor cells observed in this study represent a compensatory increase for the loss of more potent members of the HSC hierarchy.

Indexed as

AgingAntigens, CDHematopoietic Stem CellsHumansAntigens, CD

Identifiers

PMID24246745
PMCPMC3944726
OpenAlexW1980421076

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.