ArticleBiomaterials2014
Enhanced GLT-1 mediated glutamate uptake and migration of primary astrocytes directed by fibronectin-coated electrospun poly-L-lactic acid fibers.
Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 86 citations in OpenAlex.
- Advances in the research of the role of macrophage/microglia polarization-mediated inflammatory response in spinal cord injury.Frontiers in immunology · 2022Pooled it
- Electrically Active Biomaterials for Stimulation and Regeneration in Tissue Engineering.Journal of biomedical materials research. Part A · 2025Review
- Modulating neuroinflammation through molecular, cellular and biomaterial-based approaches to treat spinal cord injury.Bioengineering & translational medicine · 2023Review
- Nanomaterial payload delivery to central nervous system glia for neural protection and repair.Frontiers in cellular neuroscience · 2023Review
- The pathogenesis of DLD-mediated cuproptosis induced spinal cord injury and its regulation on immune microenvironment.Frontiers in cellular neuroscience · 2023Article
- Recent Advances in Cell and Functional Biomaterial Treatment for Spinal Cord Injury.BioMed research international · 2022Review
- Research progress, models and simulation of electrospinning technology: a review.Journal of materials science · 2022Review
- Thermosensitive collagen/fibrinogen gels loaded with decorin suppress lesion site cavitation and promote functional recovery after spinal cord injury.Scientific reports · 2021Article
- Advanced Bio-Based Polymers for Astrocyte Cell Models.Materials (Basel, Switzerland) · 2021Review
- Polymeric Fibers as Scaffolds for Spinal Cord Injury: A Systematic Review.Frontiers in bioengineering and biotechnology · 2021Review
- Electrospun Fiber Scaffolds for Engineering Glial Cell Behavior to Promote Neural Regeneration.Bioengineering (Basel, Switzerland) · 2020Review
- TGFβ3 is neuroprotective and alleviates the neurotoxic response induced by aligned poly-l-lactic acid fibers on naïve and activated primary astrocytes.Acta biomaterialia · 2020Article
- Porous Silicon Nanoparticles Embedded in Poly(lactic-Advanced functional materials · 2020Article
- Biomaterial strategies for creatingCurrent opinion in biomedical engineering · 2020Article
- The Effect of Electrospun Fiber Diameter on Astrocyte-Mediated Neurite Guidance and Protection.ACS applied bio materials · 2019Article
- Fibronectin Functionalized Electrospun Fibers by Using Benign Solvents: Best Way to Achieve Effective Functionalization.Frontiers in bioengineering and biotechnology · 2019Article
- Electrospun fiber surface nanotopography influences astrocyte-mediated neurite outgrowth.Biomedical materials (Bristol, England) · 2018Article
- Biomaterial strategies for limiting the impact of secondary events following spinal cord injury.Biomedical materials (Bristol, England) · 2018Review
- Using biomaterials to promote pro-regenerative glial phenotypes after nervous system injuries.Biomedical materials (Bristol, England) · 2018Review
- Biomaterial Approaches to Modulate Reactive Astroglial Response.Cells, tissues, organs · 2018Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Bioengineered fiber substrates are increasingly studied as a means to promote regeneration and remodeling in the injured central nervous system (CNS). Previous reports largely focused on the ability of oriented scaffolds to bridge injured regions and direct outgrowth of axonal projections. In the present work, we explored the effects of electrospun microfibers on the migration and physiological properties of brain astroglial cells. Primary rat astrocytes were cultured on either fibronectin-coated poly-L-lactic acid (PLLA) films, fibronectin-coated randomly oriented PLLA electrospun fibers, or fibronectin-coated aligned PLLA electrospun fibers. Aligned PLLA fibers strongly altered astrocytic morphology, orienting cell processes, actin microfilaments, and microtubules along the length of the fibers. On aligned fibers, astrocytes also significantly increased their migration rates in the direction of fiber orientation. We further investigated if fiber topography modifies astrocytic neuroprotective properties, namely glutamate and glutamine transport and metabolism. This was done by quantifying changes in mRNA expression (qRT-PCR) and protein levels (Western blotting) for a battery of relevant biomolecules. Interestingly, we found that cells grown on random and/or aligned fibers increased the expression levels of two glutamate transporters, GLAST and GLT-1, and an important metabolic enzyme, glutamine synthetase, as compared to the fibronectin-coated films. Functional assays revealed increases in glutamate transport rates due to GLT-1 mediated uptake, which was largely determined by the dihydrokainate-sensitive GLT-1. Overall, this study suggests that aligned PLLA fibers can promote directed astrocytic migration, and, of most importance, our in vitro results indicate for the first time that electrospun PLLA fibers can positively modify neuroprotective properties of glial cells by increasing rates of glutamate uptake.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.