ArticleThe journal of physical chemistry. B2013
Two-photon fluorescence spectroscopy and imaging of 4-dimethylaminonaphthalimide peptide and protein conjugates.
Article in The journal of physical chemistry. B, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 16 citations in OpenAlex.
- Environment-sensitive turn-on fluorescent probe enables live cell imaging of myeloperoxidase activity during NETosis.Communications chemistry · 2024Article
- Toward Point-of-Care Detection ofJACS Au · 2021Article
- Two Photon Spectroscopy Can Serve as a Marker of Protein Denaturation Pathway.Journal of fluorescence · 2018Article
- Visualizing the chain-flipping mechanism in fatty-acid biosynthesis.Angewandte Chemie (International ed. in English) · 2014Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We report detailed photophysical studies on the two-photon fluorescence processes of the solvatochromic fluorophore 4-DMN as a conjugate of the calmodulin (CaM) and the associated CaM-binding peptide M13. Strong two-photon fluorescence enhancement has been observed which is associated with calcium binding. It is found that the two-photon absorption cross-section is strongly dependent on the local environment surrounding the 4-DMN fluorophore in the CaM conjugates, providing sensitivity between sites of fluorophore attachment. Utilizing time-resolved measurements, the emission dynamics of 4-DMN under various environmental (solvent) conditions are analyzed. In addition, anisotropy measurements reveal that the 4-DMN-S38C-CaM system has restricted rotation in the calcium-bound calmodulin. To establish the utility for cellular imaging, two-photon fluorescence microscopy studies were also carried out with the 4-DMN-modified M13 peptide in cells. Together, these studies provide strong evidence that 4-DMN is a useful probe in two-photon imaging, with advantageous properties for cellular experiments.
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