Trial reportPloS one2013
High calcium bioglass enhances differentiation and survival of endothelial progenitor cells, inducing early vascularization in critical size bone defects.
Trial report in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Evaluation of the bone regeneration potential of bioactive glass in implant site development surgeries: a systematic review of the literature.Clinical oral investigations · 2015Pooled it
- Bacterial-Derived Polyhydroxyalkanoate/Bioceramic Composites in Clinical Practice: State of the Art and Future Perspectives.ACS biomaterials science & engineering · 2025Review
- A new 3D-printed polylactic acid-bioglass composite for bone tissue engineering induces angiogenesisInternational journal of bioprinting · 2023Article
- In vitro Evaluation of a 20% Bioglass-Containing 3D printable PLA Composite for Bone Tissue Engineering.International journal of bioprinting · 2022Article
- Article
- 3D-Printing of Hierarchically Designed and Osteoconductive Bone Tissue Engineering Scaffolds.Materials (Basel, Switzerland) · 2020Article
- Effectiveness of Surface Treatment with Amine Plasma for Improving the Biocompatibility of Maxillofacial Plates.Materials (Basel, Switzerland) · 2019Article
- Cultivation of EPC and co-cultivation with MSC on β-TCP granules in vitro is feasible without fibronectin coating but influenced by scaffolds' design.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2019Article
- Autologous cell-based therapy for treatment of large bone defects: from bench to bedside.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2018Review
- Tissue engineered vascularized periosteal flap enriched with MSC/EPCs for the treatment of large bone defects in rats.International journal of molecular medicine · 2017Article
- Stem cells, growth factors and scaffolds in craniofacial regenerative medicine.Genes & diseases · 2016Article
- The Possible Roles of Biological Bone Constructed with Peripheral Blood Derived EPCs and BMSCs in Osteogenesis and Angiogenesis.BioMed research international · 2016Article
- Characterization of bone marrow mononuclear cells on biomaterials for bone tissue engineering in vitro.BioMed research international · 2015Article
- Biomaterials for craniofacial bone engineering.Journal of dental research · 2014Review
- Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Early vascularization is a prerequisite for successful bone healing and endothelial progenitor cells (EPC), seeded on appropriate biomaterials, can improve vascularization. The type of biomaterial influences EPC function with bioglass evoking a vascularizing response. In this study the influence of a composite biomaterial based on polylactic acid (PLA) and either 20 or 40% bioglass, BG20 and BG40, respectively, on the differentiation and survival of EPCs in vitro was investigated. Subsequently, the effect of the composite material on early vascularization in a rat calvarial critical size defect model with or without EPCs was evaluated. Human EPCs were cultured with β-TCP, PLA, BG20 or BG40, and seeding efficacy, cell viability, cell morphology and apoptosis were analysed in vitro. BG40 released the most calcium, and improved endothelial differentiation and vitality best. This effect was mimicked by adding an equivalent amount of calcium to the medium and was diminished in the presence of the calcium chelator, EGTA. To analyze the effect of BG40 and EPCs in vivo, a 6-mm diameter critical size calvarial defect was created in rats (n = 12). Controls (n = 6) received BG40 and the treatment group (n = 6) received BG40 seeded with 5×10(5) rat EPCs. Vascularization after 1 week was significantly improved when EPCs were seeded onto BG40, compared to implanting BG40 alone. This indicates that Ca(2+) release improves EPC differentiation and is useful for enhanced early vascularization in critical size bone defects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.