ArticlePLoS genetics2013
RNA∶DNA hybrids initiate quasi-palindrome-associated mutations in highly transcribed yeast DNA.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Protein innovation through template switching in the Saccharomyces cerevisiae lineage.Scientific reports · 2021Article
- Impact of 1,The Journal of biological chemistry · 2020Article
- Human DNA polymerase η has reverse transcriptase activity in cellular environments.The Journal of biological chemistry · 2019Article
- Repeat Instability in the Fragile X-Related Disorders: Lessons from a Mouse Model.Brain sciences · 2019Article
- Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.Microbial cell (Graz, Austria) · 2019Review
- RNase H2, mutated in Aicardi-Goutières syndrome, promotes LINE-1 retrotransposition.The EMBO journal · 2018Article
- The Prevalence and Evolutionary Conservation of Inverted Repeats in Proteobacteria.Genome biology and evolution · 2018Article
- Whole Genome Sequence Analysis of Mutations Accumulated inG3 (Bethesda, Md.) · 2017Article
- Ribonucleotides and Transcription-Associated Mutagenesis in Yeast.Journal of molecular biology · 2017Review
- Article
- Genome instabilities arising from ribonucleotides in DNA.DNA repair · 2017Review
- The role of topoisomerase I in suppressing genome instability associated with a highly transcribed guanine-rich sequence is not restricted to preventing RNA:DNA hybrid accumulation.Nucleic acids research · 2016Article
- Analysis of Ribonucleotide Removal from DNA by Human Nucleotide Excision Repair.The Journal of biological chemistry · 2015Article
- Topoisomerase I alone is sufficient to produce short DNA deletions and can also reverse nicks at ribonucleotide sites.The Journal of biological chemistry · 2015Article
- Redundancy in ribonucleotide excision repair: Competition, compensation, and cooperation.DNA repair · 2015Review
- Replication and transcription on a collision course: eukaryotic regulation mechanisms and implications for DNA stability.Frontiers in genetics · 2015Review
- Ribonucleotides in DNA: origins, repair and consequences.DNA repair · 2014Review
- Transcription-associated mutagenesis.Annual review of genetics · 2014Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
RNase H enzymes promote genetic stability by degrading aberrant RNA:DNA hybrids and by removing ribonucleotide monophosphates (rNMPs) that are present in duplex DNA. Here, we report that loss of RNase H2 in yeast is associated with mutations that extend identity between the arms of imperfect inverted repeats (quasi-palindromes or QPs), a mutation type generally attributed to a template switch during DNA synthesis. QP events were detected using frameshift-reversion assays and were only observed under conditions of high transcription. In striking contrast to transcription-associated short deletions that also are detected by these assays, QP events do not require Top1 activity. QP mutation rates are strongly affected by the direction of DNA replication and, in contrast to their elevation in the absence of RNase H2, are reduced when RNase H1 is additionally eliminated. Finally, transcription-associated QP events are limited by components of the nucleotide excision repair pathway and are promoted by translesion synthesis DNA polymerases. We suggest that QP mutations reflect either a transcription-associated perturbation of Okazaki-fragment processing, or the use of a nascent transcript to resume replication following a transcription-replication conflict.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.