ArticlePLoS genetics2013
RNAi-dependent and independent control of LINE1 accumulation and mobility in mouse embryonic stem cells.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Notices and Policies for Retractions, Expressions of Concern, Errata and Corrigenda: Their Importance, Content, and Context.Science and engineering ethics · 2017Article
- Multiple Authorship in Scientific Manuscripts: Ethical Challenges, Ghost and Guest/Gift Authorship, and the Cultural/Disciplinary Perspective.Science and engineering ethics · 2016Article
- Production of small RNAs by mammalian Dicer.Pflugers Archiv : European journal of physiology · 2016Review
- Review
- miR-128 represses L1 retrotransposition by binding directly to L1 RNA.Nature structural & molecular biology · 2015Article
- RNA interference machinery-mediated gene regulation in mouse adult neural stem cells.BMC neuroscience · 2015Article
- Retraction: RNAi-Dependent and Independent Control of LINE1 Accumulation and Mobility in Mouse Embryonic Stem Cells.PLoS genetics · 2015Article
- Swiss army knives: non-canonical functions of nuclear Drosha and Dicer.Nature reviews. Molecular cell biology · 2015Review
- DNA methylation restrains transposons from adopting a chromatin signature permissive for meiotic recombination.Genes & development · 2015Article
- Expression of Concern: RNAi-Dependent and Independent Control of LINE1 Accumulation and Mobility in Mouse Embryonic Stem Cells.PLoS genetics · 2015Article
- Retrotransposons shape species-specific embryonic stem cell gene expression.Retrovirology · 2015Review
- Correction: RNAi-Dependent and Independent Control of LINE1 Accumulation and Mobility in Mouse Embryonic Stem Cells.PLoS genetics · 2015Article
- The Influence of LINE-1 and SINE Retrotransposons on Mammalian Genomes.Microbiology spectrum · 2015Review
- Comparative analysis among the small RNA populations of source, sink and conductive tissues in two different plant-virus pathosystems.BMC genomics · 2015Article
- Embryonic stem cell-specific microRNAs contribute to pluripotency by inhibiting regulators of multiple differentiation pathways.Nucleic acids research · 2014Article
- Mobile DNA elements in the generation of diversity and complexity in the brain.Nature reviews. Neuroscience · 2014Review
- Replication of many human viruses is refractory to inhibition by endogenous cellular microRNAs.Journal of virology · 2014Article
- Evolutionally dynamic L1 regulation in embryonic stem cells.Genes & development · 2014Article
- An expanding universe of the non-coding genome in cancer biology.Carcinogenesis · 2014Review
- An antisense promoter in mouse L1 retrotransposon open reading frame-1 initiates expression of diverse fusion transcripts and limits retrotransposition.Nucleic acids research · 2014Article
Corrections and comments
- Correction · 2015-05-14Error in Image · · Olivier Voinnet has finding of scientific misconduct http://www2.cnrs.fr/en/2593.htm
- Expression of concern · 2015-06-29Error in Analyses · Error in Image · Investigation by Journal/Publisher · · Olivier Voinnet has finding of scientific misconduct http://www2.cnrs.fr/en/2593.htm
- Retraction · 2015-09-03Error in Image · Error in Methods · Investigation by Journal/Publisher · · Olivier Voinnet has finding of scientific misconduct http://www2.cnrs.fr/en/2593.htm
- Commented on by
- Erratum issued
- Expression of concern
- Retracted
Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In most mouse tissues, long-interspersed elements-1 (L1s) are silenced via methylation of their 5'-untranslated regions (5'-UTR). A gradual loss-of-methylation in pre-implantation embryos coincides with L1 retrotransposition in blastocysts, generating potentially harmful mutations. Here, we show that Dicer- and Ago2-dependent RNAi restricts L1 accumulation and retrotransposition in undifferentiated mouse embryonic stem cells (mESCs), derived from blastocysts. RNAi correlates with production of Dicer-dependent 22-nt small RNAs mapping to overlapping sense/antisense transcripts produced from the L1 5'-UTR. However, RNA-surveillance pathways simultaneously degrade these transcripts and, consequently, confound the anti-L1 RNAi response. In Dicer(-/-) mESC complementation experiments involving ectopic Dicer expression, L1 silencing was rescued in cells in which microRNAs remained strongly depleted. Furthermore, these cells proliferated and differentiated normally, unlike their non-complemented counterparts. These results shed new light on L1 biology, uncover defensive, in addition to regulatory roles for RNAi, and raise questions on the differentiation defects of Dicer(-/-) mESCs.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.