ArticleMethods in molecular biology (Clifton, N.J.)2014
Genetic engineering and chemical conjugation of potato virus X.
Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- Multivalent Display of Antimicrobial Peptides on Plant Virus Scaffolds Enhances Killing of Drug-Resistant Bacteria.ACS nano · 2026Article
- Engineering filamentous potato virus X as a platform nanotechnology for nucleic acid gene delivery.Scientific reports · 2025Article
- Plant Virus Intratumoral Immunotherapy with CPMV and PVX Elicits Durable Antitumor Immunity in a Mouse Model of Diffuse Large B-Cell Lymphoma.Molecular pharmaceutics · 2024Article
- Plant virus-derived nanoparticles decorated with genetically encoded SARS-CoV-2 nanobodies display enhanced neutralizing activity.Plant biotechnology journal · 2024Article
- Plant-Produced Viral Nanoparticles as a Functionalized Catalytic Support for Metabolic Engineering.Plants (Basel, Switzerland) · 2024Article
- Engineering Alkaline-Stable Barley Stripe Mosaic Virus-Like Particles for Efficient Surface Modification.Biochemical engineering journal · 2023Article
- Plug-and-Display Photo-Switchable Systems on Plant Virus Nanoparticles.Biotech (Basel (Switzerland)) · 2022Article
- Article
- Advanced Fusion Strategies for the Production of Functionalized Potato Virus X Virions.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The pharmacology of plant virus nanoparticles.Virology · 2021Review
- Analysis of Engineered Tobacco Mosaic Virus and Potato Virus X Nanoparticles as Carriers for Biocatalysts.Frontiers in plant science · 2021Article
- Affinity of plant viral nanoparticle potato virus X (PVX) towards malignant B cells enables cancer drug delivery.Biomaterials science · 2020Article
- Structure of Turnip mosaic virus and its viral-like particles.Scientific reports · 2019Article
- Virus-Incorporated Biomimetic Nanocomposites for Tissue Regeneration.Nanomaterials (Basel, Switzerland) · 2019Review
- Biodistribution of Filamentous Plant Virus Nanoparticles: Pepino Mosaic Virus versus Potato Virus X.Biomacromolecules · 2019Article
- Small, Smaller, Nano: New Applications for Potato Virus X in Nanotechnology.Frontiers in plant science · 2019Review
- Cooperative colloidal self-assembly of metal-protein superlattice wires.Nature communications · 2017Article
- Combination of Plant Virus Nanoparticle-Based in Situ Vaccination with Chemotherapy Potentiates Antitumor Response.Nano letters · 2017Article
- Potato virus X, a filamentous plant viral nanoparticle for doxorubicin delivery in cancer therapy.Nanoscale · 2017Article
- Adoption of the 2A Ribosomal Skip Principle to Tobacco Mosaic Virus for Peptide Display.Frontiers in plant science · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Here we report the genetic engineering and chemical modification of potato virus X (PVX) for the presentation of various peptides, proteins, and fluorescent dyes, or other chemical modifiers. Three different ways of genetic engineering are described and by these means, peptides are successfully expressed not only when the foot and mouth disease virus (FMDV) 2A sequence or a flexible glycine-serine linker is included, but also when the peptide is fused directly to the PVX coat protein. When larger proteins or unfavorable peptide sequences are presented, a partial fusion via the FMDV 2A sequence is preferable. When these PVX chimeras retain the ability to assemble into viral particles and are thus able to infect plants systemically, they can be utilized to inoculate susceptible plants for isolation of sufficient amounts of virus particles for subsequent chemical modification. Chemical modification is required for the display of nonbiological ligands such as fluorophores, polymers, and small drug compounds. We present three methods of chemical bioconjugation. For direct conjugation of small chemical modifiers to solvent exposed lysines, N-hydroxysuccinimide chemistry can be applied. Bio-orthogonal reactions such as copper-catalyzed azide-alkyne cycloaddition or hydrazone ligation are alternatives to achieve more efficient conjugation (e.g., when working with high molecular weight or insoluble ligands). Furthermore, hydrazone ligation offers an attractive route for the introduction of pH-cleavable cargos (e.g., therapeutic molecules).
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Registered trials
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