ArticleJournal of fluorescence1992
Calcium imaging using fluorescence lifetimes and long-wavelength probes.
Article in Journal of fluorescence, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 93 citations in OpenAlex.
- Fluorescence-lifetime optical electrophysiology in contracting cardiomyocytes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The promise and peril of comparing fluorescence lifetime in biology revealed by simulations.eLife · 2025Article
- The promise and peril of comparing fluorescence lifetime in biology revealed by simulations.bioRxiv : the preprint server for biology · 2025Article
- Applying first & second harmonic spectral phasor analysis on a single-wavelength calcium fluorophore.Biochemistry and biophysics reports · 2025Article
- Quantitative, Dynamic Detection of Neuronal NaBio-protocol · 2025Article
- Future applications of fluorescence lifetime imaging ophthalmoscopy in neuro-ophthalmology, neurology, and neurodegenerative conditions.Frontiers in neurology · 2025Review
- Fast and slow: Recording neuromodulator dynamics across both transient and chronic time scales.Science advances · 2024Article
- Article
- Calcite-accumulating large sulfur bacteria of the genus Achromatium in Sippewissett Salt Marsh.The ISME journal · 2015Article
- Asante Calcium Green and Asante Calcium Red--novel calcium indicators for two-photon fluorescence lifetime imaging.PloS one · 2014Article
- Phasor imaging with a widefield photon-counting detector.Journal of biomedical optics · 2012Article
- 3D-resolved investigation of the pH gradient in artificial skin constructs by means of fluorescence lifetime imaging.Pharmaceutical research · 2005Article
- Fluorescence lifetime-resolved pH imaging of living cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2003Article
- Texture analysis of fluorescence lifetime images of nuclear DNA with effect of fluorescence resonance energy transfer.Cytometry · 2001Article
- Fluorescence lifetime imaging of nuclear DNA: effect of fluorescence resonance energy transfer.Cytometry · 2000Article
- Potassium and sodium measurements at clinical concentrations using phase-modulation fluorometry.Sensors and actuators. B, Chemical · 1999Article
- Lifetime-based pH sensors: indicators for acidic environments.Analytical biochemistry · 1999Article
- Polarization sensing with visual detection.Analytical chemistry · 1999Article
- Anisotropy-based sensing with reference fluorophores.Analytical biochemistry · 1999Article
- High Throughput Screening with Multiphoton Excitation.Journal of biomolecular screening · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
We describe imaging of calcium concentrations using the long-wavelength Ca(2+) indicators, Calcium Green, Orange, and Crimson. The lifetimes of these probes were measured using the frequency-domain method and were found to increase from 50% to severalfold in response to calcium. The two-dimensional images of the calcium concentration were obtained using a new apparatus for fluorescence lifetime imaging (FLIM). We also describe procedures to correct for the position-dependent frequency response of the gain-modulated image intensifier used in the FLIM apparatus. Importantly, the FLIM method does not require the probe to display shifts in the excitation or emission spectra. Using the FLIM method, calcium imaging is possible using probes which display changes in lifetime in response to calcium. Consequently, calcium imaging is possible with excitation wavelengths ranging from 488 to as long as 620 nm, where autofluorescence and/or photochemical damage is minimal. These probes are also suitable for calcium measurements of single cells using lifetime-based flow cytometry.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.