ArticleParasitology research2014
Extracellular vesicles shed by Trypanosoma cruzi are linked to small RNA pathways, life cycle regulation, and susceptibility to infection of mammalian cells.
Article in Parasitology research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
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104 citing papers in PubMed, 156 citations in OpenAlex.
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- An Argonaute protein traffics from nematode to mouse and is a vaccine against parasitic nematodes.EMBO reports · 2026Article
- Non-Coding RNAs as Emerging Biomarkers in Leishmaniasis and Chagas Disease.Tropical medicine and infectious disease · 2025Review
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- Extracellular Vesicles Mediate Epimastigogenesis inLife (Basel, Switzerland) · 2025Article
- Extracellular Vesicles Derived from Trypanosomatids: The Key to Decoding Host-Parasite Communication.International journal of molecular sciences · 2025Review
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- tRNA-derived small RNAs (tsRNAs) in cardiovascular diseases: biogenesis, functions, and therapeutic targets.Frontiers in cardiovascular medicine · 2025Review
- Outer membrane vesicles in gram-negative bacteria and its correlation with pathogenesis.Frontiers in immunology · 2025Review
- An in-depth exploration of the multifaceted roles of EVs in the context of pathogenic single-cell microorganisms.Microbiology and molecular biology reviews : MMBR · 2024Review
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- The End Justifies the Means: Chagas Disease from a Perspective of the Host-Life (Basel, Switzerland) · 2024Review
- Predictive Role of Cluster Bean (Genes · 2024Article
- Paracrine Signaling Mediated by the Cytosolic Tryparedoxin Peroxidase ofPathogens (Basel, Switzerland) · 2024Article
- Editorial: Non-coding RNA elements as regulators of host-pathogen interactions.Frontiers in genetics · 2024Article
- RNA-containing extracellular vesicles in infection.RNA biology · 2024Review
- Guidelines for the purification and characterization of extracellular vesicles of parasites.Journal of extracellular biology · 2023Article
- Extracellular Vesicles and Their Impact on the Biology of Protozoan Parasites.Tropical medicine and infectious disease · 2023Review
- The Molecular Mechanisms Employed by the ParasiteInternational journal of molecular sciences · 2023Article
44 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The protozoan parasite Trypanosoma cruzi has a complex life cycle characterized by intracellular and extracellular forms alternating between invertebrate and mammals. To cope with these changing environments, T. cruzi undergoes rapid changes in gene expression, which are achieved essentially at the posttranscriptional level. At present, expanding families of small RNAs are recognized as key players in novel forms of posttranscriptional gene regulation in most eukaryotes. However, T. cruzi lacks canonical small RNA pathways. In a recent work, we reported the presence of alternate small RNA pathways in T. cruzi mainly represented by a homogeneous population of tRNA-derived small RNAs (tsRNAs). In T. cruzi epimastigotes submitted to nutrient starvation, tsRNAs colocalized with an argonaute protein distinctive of trypanosomatids (TcPIWI-tryp) and were recruited to particular cytoplasmic granules. Using epifluorescence and electronic microscopy, we observed that tsRNAs and the TcPIWI-tryp protein were recruited mainly to reservosomes and other intracellular vesicles including endosome-like vesicles and vesicular structures resembling the Golgi complex. These data suggested that, in T. cruzi, tsRNA biogenesis is probably part of endocytic/exocytic routes. We also demonstrated that epimastigotes submitted to nutrient starvation shed high levels of vesicles to the extracellular medium, which carry small tRNAs and TcPIWI-tryp proteins as cargo. At least a fraction of extracellular vesicle cargo was transferred between parasites and to mammalian susceptible cells. Our data afford experimental evidence, indicating that extracellular vesicles shed by T. cruzi promote not only life cycle transition of epimastigotes to trypomastigote forms but also infection susceptibility of mammalian cells.
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