ArticleNature chemical biology2014
Direct evidence for a covalent ene adduct intermediate in NAD(P)H-dependent enzymes.
Article in Nature chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Electroenzymatic COAngewandte Chemie (International ed. in English) · 2026Review
- Infrared spectroscopy reveals metal-independent carbonic anhydrase activity in crotonyl-CoA carboxylase/reductase.Chemical science · 2024Article
- From Protein Film Electrochemistry to Nanoconfined Enzyme Cascades and the Electrochemical Leaf.Chemical reviews · 2023Review
- Enzymatic Conversion of COChemical reviews · 2023Review
- NADP(H)-dependent biocatalysis without adding NADP(H).Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Intersubunit Coupling Enables Fast COACS central science · 2022Article
- Bacterial Enoyl-Reductases: The Ever-Growing List of Fabs, Their Mechanisms and Inhibition.Frontiers in microbiology · 2022Review
- Structural and catalytic characterization of Blastochloris viridis and Pseudomonas aeruginosa homospermidine synthases supports the essential role of cation-π interaction.Acta crystallographica. Section D, Structural biology · 2021Article
- Exploiting Electrode Nanoconfinement to Investigate the Catalytic Properties of Isocitrate Dehydrogenase (IDH1) and a Cancer-Associated Variant.The journal of physical chemistry letters · 2021Article
- "NAD-display": Ultrahigh-Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry.Angewandte Chemie (International ed. in English) · 2021Article
- How the Destabilization of a Reaction Intermediate Affects Enzymatic Efficiency: The Case of Human Transketolase.ACS catalysis · 2020Article
- Four amino acids define the COProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Awakening the Sleeping Carboxylase Function of Enzymes: Engineering the Natural COJournal of the American Chemical Society · 2019Article
- The expanding world of biosynthetic pericyclases: cooperation of experiment and theory for discovery.Natural product reports · 2019Review
- InhA, the enoyl-thioester reductase fromThe Journal of biological chemistry · 2018Article
- Inhibition of homoserine dehydrogenase by formation of a cysteine-NAD covalent complex.Scientific reports · 2018Article
- Structural basis for substrate specificity of methylsuccinyl-CoA dehydrogenase, an unusual member of the acyl-CoA dehydrogenase family.The Journal of biological chemistry · 2018Article
- A short history of RubisCO: the rise and fall (?) of Nature's predominant COCurrent opinion in biotechnology · 2018Review
- A conserved threonine prevents self-intoxication of enoyl-thioester reductases.Nature chemical biology · 2017Article
- Oxidative Cyclization in Natural Product Biosynthesis.Chemical reviews · 2017Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pyridine nucleotides NADH and NADPH (NAD(P)H) are ubiquitous redox coenzymes that are present in all living cells. Although about 16% of all characterized enzymes use pyridine nucleotides as hydride donors or acceptors during catalysis, a detailed understanding of how the hydride is transferred between NAD(P)H and the corresponding substrate is lacking for many enzymes. Here we present evidence for a new mechanism that operates during enzymatic hydride transfers using crotonyl-CoA carboxylase/reductase (Ccr) as a case study. We observed a covalent ene intermediate between NADPH and the substrate, crotonyl-CoA, using NMR, high-resolution MS and stopped-flow spectroscopy. Preparation of the ene intermediate further allowed direct access to the catalytic cycle of other NADPH-dependent enzymes-including those from type II fatty acid biosynthesis-in an unprecedented way, suggesting that formation of NAD(P)H ene intermediates is a more general principle in catalysis.
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