ArticleNature methods2014
A holidic medium for Drosophila melanogaster.
Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 203 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
203 citing papers in PubMed, 1 synthesis or guideline pooled it, 388 citations in OpenAlex.
- Meta-analysis of Diets Used inG3 (Bethesda, Md.) · 2020Pooled it
- Article
- Direct Pharmacological Activation of AMPK Extends Lifespan in Yeast, Worms and Flies.Aging cell · 2026Article
- Article
- High-protein diet promotes aging by activating the CG6415/AMT gene and disrupting mitochondrial homeostasis.Journal of advanced research · 2026Article
- A Landscape ofBiology · 2026Review
- Cytoplasmic fluidity couples nutrient availability and enterocyte fate in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Nuclear Factor Y controls nutrient-adaptive epithelial growth by regulating mTOR in the Drosophila midgut.Development (Cambridge, England) · 2026Article
- Gut Metabolism Links Precision Nutrition, Exercise, and Healthspan in Drosophila melanogaster.Aging cell · 2026Article
- Mating-dependent lifespan cost of sterol depletion in maleProceedings of the National Academy of Sciences of the United States of America · 2026Article
- A flexible synthetic diet platform for Drosophila nutrigenomics.Genes & nutrition · 2026Article
- Lysine Triggers Acute Oviposition by Activating the 20E-ETH-JH Signaling Cascade inInternational journal of molecular sciences · 2026Article
- Renal coenzyme A (CoA) production from VB5 fuels stem cell proliferation and tumor growth.Nature communications · 2026Article
- Disentangling the reproductive and metabolic transcriptional responses to diet in Drosophila melanogaster.G3 (Bethesda, Md.) · 2026Article
- Ovary-Derived Signals Align Protein Appetite with Oogenesis.bioRxiv : the preprint server for biology · 2026Article
- Short lifespan under dietary cholesterol depletion is associated with gut dysfunction in Drosophila melanogaster females.npj aging · 2026Article
- Metabolic Alterations Induced by a Seizure-Causing Sodium Channel Mutation and their Partial Normalization by Dietary α-Linolenic Acid in Drosophila.Neurochemical research · 2026Article
- The Fruit Fly Auxodrome: a computer vision setup for longitudinal studies of Drosophila development.Genetics · 2026Article
- Altered mitochondrial functionality in metabolic disorders: insights fromFrontiers in endocrinology · 2026Review
- Article
143 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
14 authors at 6 institutions in 5 countries.
Funding
Abstract
A critical requirement for research using model organisms is a well-defined and consistent diet. There is currently no complete chemically defined (holidic) diet available for Drosophila melanogaster. We describe a holidic medium that is equal in performance to an oligidic diet optimized for adult fecundity and lifespan. This holidic diet supports development over multiple generations but at a reduced rate. Over 7 years of experiments, the holidic diet yielded more consistent experimental outcomes than did oligidic food for egg laying by females. Nutrients and drugs were more available to flies in holidic medium and, similar to dietary restriction on oligidic food, amino acid dilution increased fly lifespan. We used this holidic medium to investigate amino acid-specific effects on food-choice behavior and report that folic acid from the microbiota is sufficient for Drosophila development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.