ArticleExperimental cell research2014
Biomimetic scaffold combined with electrical stimulation and growth factor promotes tissue engineered cardiac development.
Article in Experimental cell research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Hydrogel microsphere stem cell encapsulation enhances cardiomyocyte differentiation and functionality in scalable suspension system.Bioactive materials · 2025Article
- Fusogenic Coiled-Coil Peptides Enhance Lipid Nanoparticle-Mediated mRNA Delivery upon Intramyocardial Administration.ACS nano · 2023Article
- A Stretchable and Flexible Cardiac Tissue-Electronics Hybrid Enabling Multiple Drug Release, Sensing, and Stimulation.Small (Weinheim an der Bergstrasse, Germany) · 2019Article
- Extracellular matrix remodelling induced by alternating electrical and mechanical stimulations increases the contraction of engineered skeletal muscle tissues.Scientific reports · 2019Article
- Fabrication and characterization of electrospun PLLA/PANI/TSA fibers.RSC advances · 2019Article
- Combined treatment with electrical stimulation and insulin-like growth factor-1 promotes bone regeneration in vitro.PloS one · 2018Article
- Elastic, silk-cardiac extracellular matrix hydrogels exhibit time-dependent stiffening that modulates cardiac fibroblast response.Journal of biomedical materials research. Part A · 2016Article
- Multi-Material Tissue Engineering Scaffold with Hierarchical Pore Architecture.Advanced functional materials · 2016Article
- Electrical and mechanical stimulation of cardiac cells and tissue constructs.Advanced drug delivery reviews · 2016Review
- Clinical applications of naturally derived biopolymer-based scaffolds for regenerative medicine.Annals of biomedical engineering · 2015Review
- Physical developmental cues for the maturation of human pluripotent stem cell-derived cardiomyocytes.Stem cell research & therapy · 2014Review
- Acquisition of a quantitative, stoichiometrically conserved ratiometric marker of maturation status in stem cell-derived cardiac myocytes.Stem cell reports · 2014Article
- Human iPSC-derived cardiomyocytes and tissue engineering strategies for disease modeling and drug screening.Biotechnology advancesReview
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Toward developing biologically sound models for the study of heart regeneration and disease, we cultured heart cells on a biodegradable, microfabricated poly(glycerol sebacate) (PGS) scaffold designed with micro-structural features and anisotropic mechanical properties to promote cardiac-like tissue architecture. Using this biomimetic system, we studied individual and combined effects of supplemental insulin-like growth factor-1 (IGF-1) and electrical stimulation (ES). On culture day 8, all tissue constructs could be paced and expressed the cardiac protein troponin-T. IGF-1 reduced apoptosis, promoted cell-to-cell connectivity, and lowered excitation threshold, an index of electrophysiological activity. ES promoted formation of tissue-like bundles oriented in parallel to the electrical field and a more than ten-fold increase in matrix metalloprotease-2 (MMP-2) gene expression. The combination of IGF-1 and ES increased 2D projection length, an index of overall contraction strength, and enhanced expression of the gap junction protein connexin-43 and sarcomere development. This culture environment, designed to combine cardiac-like scaffold architecture and biomechanics with molecular and biophysical signals, enabled functional assembly of engineered heart muscle from dissociated cells and could serve as a template for future studies on the hierarchy of various signaling domains relative to cardiac tissue development.
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Registered trials
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