Evidence map›Paper›PMID 24239588›Full record

ArticleThe Journal of infection2014

Neutralization of HIV subtypes A and D by breast milk IgG from women with HIV infection in Uganda.

Jana M Palaia, Michelle McConnell, Jenna E Achenbach, Claire E Gustafson, Kristina A Stoermer, Monica Nolan, Laura A Guay, Thomas K Leitner, Flavia Matovu, Allan W Taylor and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infection, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Jana M PalaiaMucosal and Vaccine Research Colorado (MAVRC), University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA; Denver Veterans Affairs Medical Center, Denver, CO 80220, USA.
Michelle McConnellCenters for Disease Control and Prevention, Atlanta, GA 30333, USA.
Jenna E AchenbachMucosal and Vaccine Research Colorado (MAVRC), University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Claire E GustafsonMucosal and Vaccine Research Colorado (MAVRC), University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA; Denver Veterans Affairs Medical Center, Denver, CO 80220, USA.
Kristina A StoermerMucosal and Vaccine Research Colorado (MAVRC), University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Monica NolanCenters for Disease Control and Prevention, Atlanta, GA 30333, USA.
Laura A GuayMakerere University - Johns Hopkins University, Kampala, Uganda.
Thomas K LeitnerLos Alamos National Laboratory, Los Alamos, NM 87544, USA.
Flavia MatovuMakerere University - Johns Hopkins University, Kampala, Uganda.
Allan W TaylorCenters for Disease Control and Prevention, Atlanta, GA 30333, USA.
Mary Glenn FowlerMakerere University - Johns Hopkins University, Kampala, Uganda.
Edward N JanoffMucosal and Vaccine Research Colorado (MAVRC), University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA; Denver Veterans Affairs Medical Center, Denver, CO 80220, USA. Electronic address: Edward.Janoff@ucdenver.edu.
Centers for Disease Control and Prevention · USDenver VA Medical Center · USMUJHU Research Collaboration · UGUniversity of Colorado Anschutz Medical Campus · USLos Alamos National Laboratory · US

Funding

University of Colorado Cancer Center Support Grant - Lung Cancer Patient-Derived Xenografts with Autologous Human Immune SystemsP30CA046934 · NCI · UNIVERSITY OF COLORADO DENVER · PI James V Degregori · 1988 to 2026
$117.0M
HIV-1 Evolution and Functional Correlates of MTCTR01AI097265 · NIAID · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI JANOFF, EDWARD N, LUZURIAGA, KATHERINE F · 2012 to 2016
$3.9M
Mucosal Determinants of HIV Infection of Infants by Breast MilkR01HD059527 · NICHD · UNIVERSITY OF COLORADO DENVER · PI JANOFF, EDWARD N · 2009 to 2012
$2.2M
Mucosal Immune Ontogeny and Intestinal Microbiota in InfantsR21AI083615 · NIAID · UNIVERSITY OF COLORADO DENVER · PI JANOFF, EDWARD N · 2009 to 2010
$458k
NCI NIH HHS P30 CA046934NIAID NIH HHS R01 AI097265NIAID NIH HHS R01 AI41361NIAID NIH HHS R21 AI083615NICHD NIH HHS R01 HD059527
6 · The paper itself

Abstract

objectivesAmong HIV-exposed infants in resource-limited countries, 8-12% are infected postnatally by breastfeeding. However, most of those uninfected at birth remain uninfected over time despite daily exposure to HIV in breast milk. Thus, we assessed the HIV-inhibitory activity of breast milk.

methodsWe measured cross-clade neutralization in activated PBMC of Ugandan subtype A (92UG031) and D (92UG005) primary HIV by breast milk or purified milk IgG and IgA from 25 HIV-infected Ugandan women. Isotype-specific antigen recognition was resolved by immunoblot. We determined HIV subtype from envelope population sequences in cells from 13 milk samples by PCR.

resultsMilk inhibited p24 production by ≥50% (dose-dependent) by subtype A (21/25; 84%) and subtype D (11/25; 44%). IgG consistently reacted with multiple HIV antigens, including gp120/gp41, but IgA primarily recognized p24 alone. Depletion of IgG (n = 5), not IgA, diminished neutralization (mean 78 ± 33%) that was largely restored by IgG repletion. Mothers infected with subtype A more effectively neutralized subtype A than D.

conclusionsBreast milk from HIV-infected women showed homotypic and cross-subtype neutralization of HIV by IgG-dependent and -independent mechanisms. These data direct further investigations into mechanisms of resistance against postnatal transmission of HIV to infants from their mothers.

Indexed as

AdultAmino Acid SequenceAntibodies, NeutralizingAntibody SpecificityCohort StudiesFemaleHIV-1HIV InfectionsHumansImmunoglobulin GMilk, HumanMolecular Sequence DataNeutralization TestsSequence AlignmentUgandaYoung AdultAntibodies, NeutralizingImmunoglobulin GBreast milkHIVIgG, IgAMucosal immunityNeutralizationSubtype ASubtype DUganda

Identifiers

PMID24239588
PMCPMC6355459
OpenAlexW2045886955

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.