ArticleInsect molecular biology2014
MicroRNA levels are modulated in Aedes aegypti after exposure to Dengue-2.
Article in Insect molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it.
- microRNA profiles and functions in mosquitoes.PLoS neglected tropical diseases · 2018Pooled it
- Temporal and spatial profiling of Aedes albopictus immune responses to chikungunya virus infection.PLoS neglected tropical diseases · 2025Article
- Acute bee paralysis virus regulation of microRNA expression dynamics in the insect host (Frontiers in cellular and infection microbiology · 2025Article
- Dengue virus NS1 hits hard at the barrier integrity of human cerebral microvascular endothelial cells via cellular microRNA dysregulations.Tissue barriers · 2025Article
- Response of the mosquito immune system and symbiotic bacteria to pathogen infection.Parasites & vectors · 2024Review
- Extracellular vesicles secreted by Brugia malayi microfilariae modulate the melanization pathway in the mosquito host.Scientific reports · 2023Article
- Co-infection of dengue and Zika viruses mutually enhances viral replication in the mosquito Aedes aegypti.Parasites & vectors · 2023Article
- Insect-Specific Chimeric Viruses Potentiated Antiviral Responses and Inhibited Pathogenic Alphavirus Growth in Mosquito Cells.Microbiology spectrum · 2023Article
- Intrinsic factors driving mosquito vector competence and viral evolution: a review.Frontiers in cellular and infection microbiology · 2023Review
- The Involvement of Atlastin in Dengue Virus andMicrobiology spectrum · 2022Article
- MicroRNAs and other small RNAs in Aedes aegypti saliva and salivary glands following chikungunya virus infection.Scientific reports · 2022Article
- Regulatory Role of Host MicroRNAs in Flaviviruses Infection.Frontiers in microbiology · 2022Review
- Transgenic refractory Aedes aegypti lines are resistant to multiple serotypes of dengue virus.Scientific reports · 2021Article
- Small RNA Response to Infection of the Insect-Specific Lammi Virus and Hanko Virus in anViruses · 2021Article
- Vector competence of Aedes aegypti and screening for differentially expressed microRNAs exposed to Zika virus.Parasites & vectors · 2021Article
- Aedes aegypti miRNA-33 modulates permethrin induced toxicity by regulating VGSC transcripts.Scientific reports · 2021Article
- Profile of Small RNAs, vDNA Forms and Viral Integrations in Late Chikungunya Virus Infection ofViruses · 2021Article
- A Review:Vaccines · 2021Review
- Development of miRNA-Based Approaches to Explore the Interruption of Mosquito-Borne Disease Transmission.Frontiers in cellular and infection microbiology · 2021Review
- Intracellular Interactions Between Arboviruses andFrontiers in cellular and infection microbiology · 2021Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
To define microRNA (miRNA) involvement during arbovirus infection of Aedes aegypti, we mined deep sequencing libraries of Dengue type 2 (DENV2)-exposed mosquitoes. Three biological replicates for each timepoint [2, 4 and 9 days post-exposure (dpe)] and treatment group allowed us to remove the outliers associated with sample-to-sample variability. Using edgeR (R Bioconductor), designed for use with replicate deep sequencing data, we determined the log fold-change (logFC) of miRNA levels [18-23 nucleotides (nt)]. The number of significantly modulated miRNAs increased from ≤ 5 at 2 and 4 dpe to 23 unique miRNAs by 9 dpe. Putative miRNA targets were predicted by aligning miRNAs to the transcriptome, and the list was reduced to include the intersection of hits found using the Miranda, PITA, and TargetScan algorithms. To further reduce false-positives, putative targets were validated by cross-checking them with mRNAs reported in recent DENV2 host response transcriptome reports; 4076 targets were identified. Of these, 464 gene targets have predicted miRNA-binding sites in 3' untranslated regions. Context-specific target functional groups include proteins involved in transport, transcriptional regulation, mitochondrial function, chromatin modification and signal transduction processes known to be required for viral replication and dissemination. The miRNA response is placed in context with other vector host response studies by comparing the predicted targets with those of transcriptome studies. Together, these data are consistent with the hypothesis that profound and persistent changes to gene expression occur in DENV2-exposed mosquitoes.
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