ArticleAnalytical chemistry2013
Measuring protein-ligand interactions using liquid sample desorption electrospray ionization mass spectrometry.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Perturbation-Modulated Native Mass Spectrometry Excludes a Nonspecific Drug Target Protein Binder Based on Conformation Stability Change.Analytical chemistry · 2025Article
- Drug target discovery by magnetic nanoparticles coupled mass spectrometry.Journal of pharmaceutical analysis · 2021Article
- Development of a target identification approach using native mass spectrometry.Scientific reports · 2021Article
- Advances in MS Based Strategies for Probing Ligand-Target Interactions: Focus on Soft Ionization Mass Spectrometric Techniques.Frontiers in chemistry · 2019Review
- Reactive Laser Ablation Electrospray Ionization Time-Resolved Mass Spectrometry of Click Reactions.Analytical chemistry · 2018Article
- Quantification of Protein-Ligand Interactions by Laser Electrospray Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2018Article
- Native Desorption Electrospray Ionization Liberates Soluble and Membrane Protein Complexes from Surfaces.Angewandte Chemie (International ed. in English) · 2017Article
- Rapid Hydrogen-Deuterium Exchange in Liquid Droplets.Journal of the American Chemical Society · 2017Article
- On the preservation of non-covalent protein complexes during electrospray ionization.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2016Article
- Monitoring Enzymatic Reactions in Real Time Using Venturi Easy Ambient Sonic-Spray Ionization Mass Spectrometry.Analytical chemistry · 2016Article
- Influence of Sulfolane on ESI-MS Measurements of Protein-Ligand Affinities.Journal of the American Society for Mass Spectrometry · 2016Article
- Enhancing Performance of Liquid Sample Desorption Electrospray Ionization Mass Spectrometry Using Trap and Capillary Columns.International journal of mass spectrometry · 2015Article
- Rapid assessment of human amylin aggregation and its inhibition by copper(II) ions by laser ablation electrospray ionization mass spectrometry with ion mobility separation.Analytical chemistry · 2015Article
- Online Investigation of Aqueous-Phase Electrochemical Reactions by Desorption Electrospray Ionization Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2015Article
- Paper-Based Electrochemical Cell Coupled to Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2015Article
- Quantifying protein-carbohydrate interactions using liquid sample desorption electrospray ionization mass spectrometry.Journal of the American Society for Mass Spectrometry · 2015Article
- Integration of electrochemistry with ultra-performance liquid chromatography/mass spectrometry.European journal of mass spectrometry (Chichester, England) · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have previously shown that liquid sample desorption electrospray ionization-mass spectrometry (DESI-MS) is able to measure large proteins and noncovalently bound protein complexes (to 150 kDa) (Ferguson et al., Anal. Chem. 2011, 83, 6468-6473). In this study, we further investigate the application of liquid sample DESI-MS to probe protein-ligand interactions. Liquid sample DESI allows the direct formation of intact protein-ligand complex ions by spraying ligands toward separate protein sample solutions. This type of "reactive" DESI methodology can provide rapid information on binding stiochiometry, selectivity, and kinetics, as demonstrated by the binding of ribonuclease A (RNaseA, 13.7 kDa) with cytidine nucleotide ligands and the binding of lysozyme (14.3 kDa) with acetyl chitose ligands. A higher throughput method for ligand screening by liquid sample DESI was demonstrated, in which different ligands were sequentially injected as a segmented flow for DESI ionization. Furthermore, supercharging to enhance analyte charge can be integrated with liquid sample DESI-MS, without interfering with the formation of protein-ligand complexes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.