ArticlePloS one2013
Characterization of the vaginal microbiota among sexual risk behavior groups of women with bacterial vaginosis.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. It is linked to 2 registered trials, which are not on this map. Cited by 12 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Prevalence Rates of Sexually Transmitted Infections and Sexual Risk Behaviors Among African American Women Who Have Sex With Women
Pathogenesis of Bacterial Vaginosis in Women Who Have Sex With Women
Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Factors Associated with Bacterial Vaginosis among Women Who Have Sex with Women: A Systematic Review.PloS one · 2015Pooled it
- Effect of metronidazole on vaginal microbiota associated with asymptomatic bacterial vaginosis.Access microbiology · 2021Article
- Changes in the vaginal microbiota across a gradient of urbanization.Scientific reports · 2020Article
- Comparison of vaginal microbiota in gynecologic cancer patients pre- and post-radiation therapy and healthy women.Cancer medicine · 2020Article
- In Silico and Experimental Evaluation of Primer Sets for Species-Level Resolution of the Vaginal Microbiota Using 16S Ribosomal RNA Gene Sequencing.The Journal of infectious diseases · 2019Article
- Comparisons of vaginal flora patterns among sexual behaviour groups of women: implications for the pathogenesis of bacterial vaginosis.Sexual health · 2018Article
- Microbiota-based analysis reveals specific bacterial traits and a novel strategy for the diagnosis of infectious infertility.PloS one · 2018Article
- Therapeutic Opportunities in the Vaginal Microbiome.Microbiology spectrum · 2017Review
- Fetal Tissues Tested for Microbial Sterility by Culture- and PCR-Based Methods Can be Safely Used in Clinics.Cell transplantation · 2017Article
- Endophytes: A Treasure House of Bioactive Compounds of Medicinal Importance.Frontiers in microbiology · 2016Review
- Association between BVAB1 and high Nugent scores among women with bacterial vaginosis.Diagnostic microbiology and infectious disease · 2014Article
- Article
Corrections and comments
- Erratum issuedSwiatlo, Edwin [added]
Authors and funding
9 authors.
Funding
Abstract
backgroundThe pathogenesis of bacterial vaginosis (BV) remains elusive. BV may be more common among women who have sex with women (WSW). The objective of this study was to use 454 pyrosequencing to investigate the vaginal microbiome of WSW, women who have sex with women and men (WSWM), and women who have sex with men (WSM) with BV to determine if there are differences in organism composition between groups that may inform new hypotheses regarding the pathogenesis of BV.
methodsVaginal swab specimens from eligible women with BV at the Mississippi State Department of Health STD Clinic were used. After DNA extraction, 454 pyrosequencing of PCR-amplified 16S rRNA gene sequences was performed. Sequence data was classified using the Ribosomal Database Program classifer. Complete linkage clustering analysis was performed to compare bacterial community composition among samples. Differences in operational taxonomic units with an abundance of ≥ 2% between risk behavior groups were determined. Alpha and beta diversity were measured using Shannon's Index implemented in QIIME and Unifrac analysis, respectively.
results33 WSW, 35 WSWM, and 44 WSM were included. The vaginal bacterial communities of all women clustered into four taxonomic groups with the dominant taxonomic group in each being Lactobacillus, Lachnospiraceae, Prevotella, and Sneathia. Regarding differences in organism composition between risk behavior groups, the abundance of Atopobium (relative ratio (RR)=0.24; 95%CI 0.11-0.54) and Parvimonas (RR=0.33; 95%CI 0.11-0.93) were significantly lower in WSW than WSM, the abundance of Prevotella was significantly higher in WSW than WSWM (RR=1.77; 95%CI 1.10-2.86), and the abundance of Atopobium (RR=0.41; 95%CI 0.18-0.88) was significantly lower in WSWM than WSM. Overall, WSM had the highest diversity of bacterial taxa.
conclusionThe microbiology of BV among women in different risk behavior groups is heterogeneous. WSM in this study had the highest diversity of bacterial taxa. Additional studies are needed to better understand these differences.
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Registered trials
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