ArticlePloS one2013
2A and the auxin-based degron system facilitate control of protein levels in Plasmodium falciparum.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- An ER-resident lipid scramblase is crucial for biogenesis and function of apicomplexan parasite secretory organelles.Nature communications · 2025Article
- The ratio of Wnt signaling activity to Sox2 transcription factor levels predicts neuromesodermal fate potential.Development (Cambridge, England) · 2025Article
- Specific auxin and medium combinations alterbioRxiv : the preprint server for biology · 2025Article
- Protein-targeting reverse genetic approaches: the future of oocyte and preimplantation embryo research.Molecular human reproduction · 2025Review
- Specific auxin and medium combinations altermicroPublication biology · 2025Article
- Establishment of the auxin inducible degron system for Babesia duncani: a conditional knockdown tool to study precise protein regulation in Babesia spp.Parasites & vectors · 2024Article
- Generation of a new DiCre expressing parasite strain for functional characterization of Plasmodium falciparum genes in blood stages.Scientific reports · 2024Article
- Rapid depletion of target proteins in plants by an inducible protein degradation system.The Plant cell · 2024Article
- Rapid, efficient auxin-inducible protein degradation inmSphere · 2023Article
- Rapid, efficient auxin-inducible protein degradation inbioRxiv : the preprint server for biology · 2023Article
- Phosphoproteomic Approaches for Identifying Phosphatase and Kinase Substrates.Molecules (Basel, Switzerland) · 2023Review
- A toolbox for conditional control of gene expression in apicomplexan parasites.Molecular microbiology · 2022Review
- Paving the Way: Contributions of Big Data to Apicomplexan and Kinetoplastid Research.Frontiers in cellular and infection microbiology · 2022Review
- Some conditions apply: Systems for studying Plasmodium falciparum protein function.PLoS pathogens · 2021Review
- Prey for the Proteasome: Targeted Protein Degradation-A Medicinal Chemist's Perspective.Angewandte Chemie (International ed. in English) · 2020Review
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- Histone deacetylase inhibitors with high in vitro activities against Plasmodium falciparum isolates collected from Gabonese children and adults.Scientific reports · 2019Article
- Auxin-inducible protein degradation as a novel approach for protein depletion and reverse genetic discoveries in mammalian oocytes†.Biology of reproduction · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analysis of gene function in Plasmodium falciparum, the most important human malaria parasite, is restricted by the lack of robust and simple reverse genetic tools. Approaches to manipulate protein levels post-translationally are powerful tools to study protein-off effects especially in the haploid malaria parasite where genetic knockouts of essential genes are lethal. We investigated if the auxin-inducible degron system is functional in P. falciparum and found that degron-tagged yellow fluorescent protein levels were efficiently reduced upon addition of auxin which otherwise had no effect on parasite viability. The genetic components required in this conditional approach were co-expressed in P. falciparum by applying the small peptide 2A. 2A is a self-processing peptide from Foot-And-Mouth Disease virus that allows the whole conditional system to be accommodated on a single plasmid vector and ensures stoichiometric expression levels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.