ArticleThe Journal of cell biology1986
Probing the structure of cytoplasm.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 132 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
132 citing papers in PubMed, 333 citations in OpenAlex.
- Article
- Evidence that local viscosity and NOX-dependent ROS increases render the tardigrade H. exemplaris resilient to extreme physical force.bioRxiv : the preprint server for biology · 2026Article
- Compartmentalized cytoplasmic tradewinds direct soluble proteins.Nature communications · 2026Article
- Rapid Single-cell Measurement of Transient Transmembrane Water Flow under Osmotic Gradient.The Journal of membrane biology · 2026Article
- Active fluctuations of cytoplasmic actomyosin networks facilitate dynein-driven transport.iScience · 2025Article
- An Insight into Cancer Cells and Disease Progression Through the Lens of Mathematical Modeling.Current issues in molecular biology · 2025Review
- Molecular and Biophysical Perspectives on Dormancy Breaking: Lessons from Yeast Spore.Biomolecules · 2025Review
- Cytoplasmic fluidization contributes to breaking spore dormancy in fission yeast.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Size Sensitivity of Metabolite Diffusion in Macromolecular Crowds.Nano letters · 2024Article
- On the origin of the nucleus: a hypothesis.Microbiology and molecular biology reviews : MMBR · 2023Review
- How it feels in a cell.Trends in cell biology · 2023Review
- Quantifying cell viability through organelle ratiometric probing.Chemical science · 2023Article
- Vast heterogeneity in cytoplasmic diffusion rates revealed by nanorheology and Doppelgänger simulations.Biophysical journal · 2023Article
- Size- and position-dependent cytoplasm viscoelasticity through hydrodynamic interactions with the cell surface.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Partitioning of ribonucleoprotein complexes from the cellular actin cortex.Science advances · 2022Article
- Intracellular Crowding by Bio-Orthogonal Hydrogel Formation Induces Reversible Molecular Stasis.Advanced materials (Deerfield Beach, Fla.) · 2022Article
- Physical properties of the cytoplasm modulate the rates of microtubule polymerization and depolymerization.Developmental cell · 2022Article
- Contribution of cytoplasm viscoelastic properties to mitotic spindle positioning.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Functional compartmentalization of photoreceptor neurons.Pflugers Archiv : European journal of physiology · 2021Review
- The cellular mechanobiology of aging: from biology to mechanics.Annals of the New York Academy of Sciences · 2021Review
72 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have used size-fractionated, fluorescent dextrans to probe the structure of the cytoplasmic ground substance of living Swiss 3T3 cells by fluorescence recovery after photobleaching and video image processing. The data indicate that the cytoplasm of living cells has a fluid phase viscosity four times greater than water and contains structural barriers that restrict free diffusion of dissolved macromolecules in a size-dependent manner. Assuming these structural barriers comprise a filamentous meshwork, the combined fluorescence recovery after photobleaching and imaging data suggest that the average pore size of the meshwork is in the range of 300 to 400 A, but may be as small as 200 A in some cytoplasmic domains.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.