ArticleNucleic acids research2014
Cycling of the E. coli lagging strand polymerase is triggered exclusively by the availability of a new primer at the replication fork.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- DnaE uses strand displacement synthesis during Okazaki fragment repair.bioRxiv : the preprint server for biology · 2026Article
- Mechanisms of chromosomal DNA replication in Escherichia coli and Bacillus subtilis.FEMS microbiology reviews · 2026Review
- The bacteriophage T4 replisome: a model system for understanding DNA replication mechanisms.EcoSal Plus · 2025Review
- DnaB helicase dynamics in bacterial DNA replication resolved by single-molecule studies.Nucleic acids research · 2021Article
- Replication fidelity in E. coli: Differential leading and lagging strand effects for dnaE antimutator alleles.DNA repair · 2019Article
- Bacillus subtilis RarA modulates replication restart.Nucleic acids research · 2018Article
- High-accuracy lagging-strand DNA replication mediated by DNA polymerase dissociation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Understanding DNA replication by the bacteriophage T4 replisome.The Journal of biological chemistry · 2017Review
- Collision with duplex DNA renders Escherichia coli DNA polymerase III holoenzyme susceptible to DNA polymerase IV-mediated polymerase switching on the sliding clamp.Scientific reports · 2017Article
- Article
- RNA primer-primase complexes serve as the signal for polymerase recycling and Okazaki fragment initiation in T4 phage DNA replication.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- DNA Polymerase III, but Not Polymerase IV, Must Be Bound to a τ-Containing DnaX Complex to Enable Exchange into Replication Forks.The Journal of biological chemistry · 2016Article
- The DNA polymerase III holoenzyme contains γ and is not a trimeric polymerase.Nucleic acids research · 2016Article
- cryo-EM structures of theeLife · 2015Article
- Review
- Slow unloading leads to DNA-bound β2-sliding clamp accumulation in live Escherichia coli cells.Nature communications · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Two models have been proposed for triggering release of the lagging strand polymerase at the replication fork, enabling cycling to the primer for the next Okazaki fragment--either collision with the 5'-end of the preceding fragment (collision model) or synthesis of a new primer by primase (signaling model). Specific perturbation of lagging strand elongation on minicircles with a highly asymmetric G:C distribution with ddGTP or dGDPNP yielded results that confirmed the signaling model and ruled out the collision model. We demonstrated that the presence of a primer, not primase per se, provides the signal that triggers cycling. Lagging strand synthesis proceeds much faster than leading strand synthesis, explaining why gaps between Okazaki fragments are not found under physiological conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.