ArticleMethods in molecular biology (Clifton, N.J.)2014
Conditional gene-trap mutagenesis in zebrafish.
Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Fishing forward and reverse: Advances in zebrafish phenomics.Mechanisms of development · 2018Review
- Expression and function of ATP-dependent potassium channels in zebrafish islet β-cells.Royal Society open science · 2017Article
- Dynamic structure and protein expression of the live embryonic heart captured by 2-photon light sheet microscopy and retrospective registration.Biomedical optics express · 2015Article
- Interpreting human genetic variation with in vivo zebrafish assays.Biochimica et biophysica acta · 2014Review
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Authors and funding
3 authors.
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Abstract
Zebrafish has become a widely used model for analysis of gene function. Several methods have been used to create mutations in this organism and thousands of mutant lines are available. However, all the conventional zebrafish mutations affect the gene in all cells at all time, making it difficult to determine tissue-specific functions. We have adopted a FlEx Trap approach to generate conditional mutations in zebrafish by gene-trap mutagenesis. Combined with appropriate Cre or Flp lines, the insertional mutants not only allow spatial- and temporal-specific gene inactivation but also permit spatial- and temporal-specific rescue of the disrupted gene. We provide experimental details on how to generate and use such mutations.
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Registered trials
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