ArticleMethods in molecular biology (Clifton, N.J.)2014
Rapid genetic modification of mouse embryonic stem cells by Inducible Cassette Exchange recombination.
Article in Methods in molecular biology (Clifton, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Independent control of neurogenesis and dorsoventral patterning by NKX2-2.Genes & development · 2026Article
- Expression of transgenic biotin ligases in inducible neuronal murine cell lines by integration into the mHipp11 gene locus.PloS one · 2025Article
- Subcellular mRNA kinetic modeling reveals nuclear retention as rate-limiting.Molecular systems biology · 2024Article
- ETV2 and VEZF1 interaction and regulation of the hematoendothelial lineage during embryogenesis.Frontiers in cell and developmental biology · 2023Article
- Repurposing the lineage-determining transcription factor Atoh1 without redistributing its genomic binding sites.Frontiers in cell and developmental biology · 2022Article
- Article
- TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation.eLife · 2021Article
- LHPP-Mediated Histidine Dephosphorylation Suppresses the Self-Renewal of Mouse Embryonic Stem Cells.Frontiers in cell and developmental biology · 2021Article
- The transcription factor E2A drives neural differentiation in pluripotent cells.Development (Cambridge, England) · 2020Article
- TWIST1 Homodimers and Heterodimers Orchestrate Lineage-Specific Differentiation.Molecular and cellular biology · 2020Article
- In vitro capture and characterization of embryonic rosette-stage pluripotency between naive and primed states.Nature cell biology · 2020Article
- Eomes and Brachyury control pluripotency exit and germ-layer segregation by changing the chromatin state.Nature cell biology · 2019Article
- N-cadherin stabilises neural identity by dampening anti-neural signals.Development (Cambridge, England) · 2019Article
- Id1 Stabilizes Epiblast Identity by Sensing Delays in Nodal Activation and Adjusting the Timing of Differentiation.Developmental cell · 2019Article
- Retinoic Acid Induces Embryonic Stem Cells (ESCs) Transition to 2 Cell-Like State Through a Coordinated Expression ofFrontiers in cell and developmental biology · 2019Article
- RNA localization is a key determinant of neurite-enriched proteome.Nature communications · 2017Article
- The transcription factor Mesp1 interacts with cAMP-responsive element binding protein 1 (Creb1) and coactivates Ets variant 2 (Etv2) gene expression.The Journal of biological chemistry · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Embryonic stem cell (ESC) differentiation is a useful means by which to produce large quantities of cells in vitro representing early stages of embryonic development. A conditional gene expression system allows interrogation of factors at specific time points in the differentiation of ES cells to defined cell types. We have developed a method for rapidly generating conditional inducible murine ES cells by targeting genes into an Inducible Cassette Exchange (ICE) locus. The ICE locus encodes a doxycycline-inducible floxed Cre, which replaces itself with an incoming floxed gene of interest. The derivative cell lines, selected in G418, thus bear doxycycline-inducible transgenes. We provide detailed methods for performing ICE recombination and generating derivative doxycycline-inducible cell lines.
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Registered trials
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