ArticleScientific reports2013
Establishment of a novel lingual organoid culture system: generation of organoids having mature keratinized epithelium from adult epithelial stem cells.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 87 citations in OpenAlex.
- Development of a high-efficiency induction system for embryonic oral epithelium from human pluripotent stem cells.Stem cell reports · 2026Article
- Organoid Modeling of Mouse Anterior Tongue Epithelium Reveals Regional and Cellular Identities.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Advancing oral squamous cell carcinoma research: the evolving role of patient-derived cell lines, xenografts, and organoid models.3 Biotech · 2025Review
- Potential Implications of Organoids in Dental Research.Frontiers in dentistry · 2025Review
- Spatial transcriptomics reveals molecular cues underlying the site specificity of the adult mouse oral mucosa and its stem cell niches.Stem cell reports · 2024Article
- Organoids in the oral and maxillofacial region: present and future.International journal of oral science · 2024Review
- A Decade of Organoid Research: Progress and Challenges in the Field of Organoid Technology.ACS omega · 2024Review
- Perspective of 3D culture in medicine: transforming disease research and therapeutic applications.Frontiers in bioengineering and biotechnology · 2024Review
- Revolutionising oral organoids with artificial intelligence.Biomaterials translational · 2024Review
- Engineered organoids in oral and maxillofacial regeneration.iScience · 2023Review
- Therapeutic strategies of three-dimensional stem cell spheroids and organoids for tissue repair and regeneration.Bioactive materials · 2023Review
- Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids.Journal of visualized experiments : JoVE · 2022Article
- Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review.Gels (Basel, Switzerland) · 2022Review
- Application of Patient-Derived Cancer Organoids to Personalized Medicine.Journal of personalized medicine · 2022Review
- Fine-tuning of epithelial taste bud organoid to promote functional recapitulation of taste reactivity.Cellular and molecular life sciences : CMLS · 2022Article
- A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia.iScience · 2021Article
- Characterization of normal and cancer stem-like cell populations in murine lingual epithelial organoids using single-cell RNA sequencing.Scientific reports · 2021Article
- Advances in Tissue Engineering and Implications for Oral and Maxillofacial Reconstruction.Journal of the California Dental Association · 2021Article
- 3D organoids derived from the small intestine: An emerging tool for drug transport research.Acta pharmaceutica Sinica. B · 2021Review
- Immortalization and Characterization of Rat Lingual Keratinocytes in a High-Calcium and Feeder-Free Culture System Using ROCK Inhibitor Y-27632.International journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the strong need for the establishment of a lingual epithelial cell culture system, a simple and convenient culture method has not yet been established. Here, we report the establishment of a novel lingual epithelium organoid culture system using a three-dimensional matrix and growth factors. Histological analyses showed that the generated organoids had both a stratified squamous epithelial cell layer and a stratum corneum. Very recently, we showed via a multicolor lineage tracing method that Bmi1-positive stem cells exist at the base of the epithelial basal layer in the interpapillary pit. Using our new culture system, we found that organoids could be generated by single Bmi1-positive stem cells and that in the established organoids, multiple Bmi1-positive stem cells were generated at the outermost layer. Moreover, we observed that organoids harvested at an early point in culture could be engrafted and maturate in the tongue of recipient mice and that the organoids generated from carcinogen-treated mice had an abnormal morphology. Thus, this culture system presents valuable settings for studying not only the regulatory mechanisms of lingual epithelium but also lingual regeneration and carcinogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.