ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1988
Efficient regeneration of transgenic plants from rice protoplasts and correctly regulated expression of the foreign gene in the plants.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
51 citing papers in PubMed, 251 citations in OpenAlex.
- Maize transformation: history, progress, and perspectives.Molecular breeding : new strategies in plant improvement · 2021Review
- Molecular characterization, expression and functional analysis of acyl-CoA-binding protein gene family in maize (Zea mays).BMC plant biology · 2021Article
- Particle bombardment technology and its applications in plants.Molecular biology reports · 2020Review
- Agrobacterium-Mediated Genetic Transformation of Wild Oryza Species Using Immature Embryos.Rice (New York, N.Y.) · 2020Article
- Studies on growth dynamics of embryogenic cell suspension cultures of commercially important3 Biotech · 2018Article
- Acyl-CoA-binding protein family members in laticifers are possibly involved in lipid and latex metabolism of Hevea brasiliensis (the Para rubber tree).BMC genomics · 2018Article
- A novel reference plasmid for the qualitative detection of genetically modified rice in food and feed.BioMed research international · 2015Article
- The rice semi-dwarf mutant sd37, caused by a mutation in CYP96B4, plays an important role in the fine-tuning of plant growth.PloS one · 2014Article
- An efficient and high-throughput protocol for Agrobacterium-mediated transformation based on phosphomannose isomerase positive selection in Japonica rice (Oryza sativa L.).Plant cell reports · 2012Article
- Dehydration-stress-regulated transgene expression in stably transformed rice plantsPlant physiology · 1998Article
- Rice transformation: bombardment.Plant molecular biology · 1997Review
- Transformation of rice mediated by Agrobacterium tumefaciens.Plant molecular biology · 1997Review
- Evaluation of five promoters for use in transformation of oil palm (Elaeis guineensis Jacq.).Plant cell reports · 1997Article
- Screening for stable transformants and stability of β-glucuronidase gene expression in suspension cultured cells of tall fescue (Festuca arundinacea).Plant cell reports · 1996Article
- Regeneration of fertile transgenic indica (group 1) rice plants following microprojectile transformation of embryogenic suspension culture cells.Plant cell reports · 1996Article
- Selection of large quantities of embryogenic calli from indica rice seeds for production of fertile transgenic plants using the biolistic method.Plant cell reports · 1996Article
- Introduction of a chimeric gene encoding an oryzacystatin-β-glucuronidase fusion protein into rice protoplasts and regeneration of transformed plants.Plant cell reports · 1995Article
- Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts.Plant cell reports · 1995Article
- Current developments in plant biotechnology for genetic improvement: the case of rice (Oryza sativa L.).World journal of microbiology & biotechnology · 1995Article
- Expression of the rice Osgrp1 promoter-Gus reporter gene is specifically associated with cell elongation/expansion and differentiation.Plant molecular biology · 1995Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rice is one of the most important crops in the world with 35% of the total population (over two billion people) depending on it as their source of food. It is therefore essential to develop efficient methods for the transformation and regeneration of rice plants in order to delineate the exact regulatory sequences responsible for gene expression and to transfer beneficial genes into this plant. Here, for the first time, we present definitive evidence for the regeneration of a large number of transgenic rice plants after introduction of the bacterial β-glucuronidase gene into rice protoplasts. The presence of integrated copies of this gene was detected in the genome of transgenic plants by DNA hybridization analysis. Furthermore, under the control of regulatory regions from a maize alcohol dehydrogenase sequence, β-glucuronidase gene expression was detected in the roots of transgenic plants. This expression was stimulated up to six fold under anaerobic conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.