ArticleBioMed research international2013
Ouabain-induced apoptosis in cochlear hair cells and spiral ganglion neurons in vitro.
Article in BioMed research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Fasudil protects spiral ganglion neurons and hair cells against cisplatin-induced apoptosis by inhibiting reactive oxygen species accumulation and regulating the ROCK/PTEN/AKT signaling pathway.Toxicology research · 2025Article
- Otic Organoids Containing Spiral Ganglion Neuron-like Cells Derived from Human-induced Pluripotent Stem Cells as a Model of Drug-induced Neuropathy.Stem cells translational medicine · 2022Article
- Nicotine induced ototoxicity in rat cochlear organotypic cultures.Translational neuroscience · 2021Article
- Ouabain Protects Nephrogenesis in Rats Experiencing Intrauterine Growth Restriction and Partially Restores Renal Function in Adulthood.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- Cisplatin-induced vestibular hair cell lesion-less damage at high doses.Journal of otology · 2018Article
- Article
- Ouabain-induced apoptosis and inhibition of viability of tubulointerstitial cells by regulating NKA/pSrc/pERK/pAkt/pS6k/caspase 3 may contribute to lupus nephritis development.International journal of clinical and experimental pathology · 2018Article
- Kanamycin Damages Early Postnatal, but Not Adult Spiral Ganglion Neurons.Neurotoxicity research · 2017Article
- Auditory Neuropathy after Damage to Cochlear Spiral Ganglion Neurons in Mice Resulting from Conditional Expression of Diphtheria Toxin Receptors.Scientific reports · 2017Article
- The potential use of low-frequency tones to locate regions of outer hair cell loss.Hearing research · 2016Article
- Single-Cell Transcriptome Analysis of Developing and Regenerating Spiral Ganglion Neurons.Current pharmacology reports · 2016Article
- Ouabain Enhances ADPKD Cell Apoptosis via the Intrinsic Pathway.Frontiers in physiology · 2016Article
- Cobalt-Induced Ototoxicity in Rat Postnatal Cochlear Organotypic Cultures.Neurotoxicity research · 2015Article
- Apoptosis-inducing factor and calpain upregulation in glutamate-induced injury of rat spiral ganglion neurons.Molecular medicine reports · 2015Article
- Standardized surgical approaches to ear surgery in rats.Journal of otology · 2015Article
- Gene transfection mediated by polyethyleneimine-polyethylene glycol nanocarrier prevents cisplatin-induced spiral ganglion cell damage.Neural regeneration research · 2015Article
- A traditional Chinese medicine compound (Jian Er) for presbycusis in a mouse model: Reduction of apoptosis and protection of cochlear sensorineural cells and hearing.International journal of herbal medicineArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ouabain is a common tool to explore the pathophysiological changes in adult mammalian cochlea in vivo. In prior studies, locally administering ouabain via round window membrane demonstrated that the ototoxic effects of ouabain in vivo varied among mammalian species. Little is known about the ototoxic effects in vitro. Thus, we prepared cochlear organotypic cultures from postnatal day-3 rats and treated these cultures with ouabain at 50, 500, and 1000 μM for different time to elucidate the ototoxic effects of ouabain in vitro and to provide insights that could explain the comparative ototoxic effects of ouabain in vivo. Degeneration of cochlear hair cells and spiral ganglion neurons was evaluated by hair-cell staining and neurofilament labeling, respectively. Annexin V staining was used to detect apoptotic cells. A quantitative RT-PCR apoptosis-focused gene array determined changes in apoptosis-related genes. The results showed that ouabain-induced damage in vitro was dose and time dependent. 500 μM ouabain and 1000 μM ouabain were destructively traumatic to both spiral ganglion neurons and cochlear hair cells in an apoptotic signal-dependent pathway. The major apoptotic pathways in ouabain-induced spiral ganglion neuron apoptosis culminated in the stimulation of the p53 pathway and triggering of apoptosis by a network of proapoptotic signaling pathways.
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