ArticleStem cells international2013
Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC.
Article in Stem cells international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 47 citations in OpenAlex.
- Mesenchymal Stem Cell Transplantation in Type 1 Diabetes Treatment: Current Advances and Future Opportunity.Current stem cell research & therapy · 2024Review
- Trustworthy in silico cell labeling via ensemble-based image translation.Biophysical reports · 2023Article
- Characterization of Human, Ovine and Porcine Mesenchymal Stem Cells from Bone Marrow: Critical In Vitro Comparison with Regard to Humans.Life (Basel, Switzerland) · 2023Article
- Donor variability of ovine bone marrow derived mesenchymal stem cell - implications for cell therapy.International journal of veterinary science and medicine · 2023Article
- Biodistribution of allogenic umbilical cord-derived mesenchymal stromal cells after fetal repair of myelomeningocele in an ovine model.Stem cell research & therapy · 2022Article
- Clinical translation of a patient-specific scaffold-guided bone regeneration concept in four cases with large long bone defects.Journal of orthopaedic translation · 2022Article
- Mesenchymal stem cells are prospective novel off-the-shelf wound management tools.Drug delivery and translational research · 2022Review
- Investigating heterogeneities of live mesenchymal stromal cells using AI-based label-free imaging.Scientific reports · 2021Article
- Cell Sources for Human In vitro Bone Models.Current osteoporosis reports · 2021Review
- Convergence of scaffold-guided bone regeneration and RIA bone grafting for the treatment of a critical-sized bone defect of the femoral shaft.European journal of medical research · 2020Article
- Biological Characteristics and Osteogenic Differentiation of Ovine Bone Marrow Derived Mesenchymal Stem Cells Stimulated with FGF-2 and BMP-2.International journal of molecular sciences · 2020Article
- Pressure Stimuli Improve the Proliferation of Wharton's Jelly-Derived Mesenchymal Stem Cells under Hypoxic Culture Conditions.International journal of molecular sciences · 2020Article
- Characterization and Comparison of Human and Ovine Mesenchymal Stromal Cells from Three Corresponding Sources.International journal of molecular sciences · 2020Article
- Article
- Experimental Strategies of Mesenchymal Stem Cell Propagation: Adverse Events and Potential Risk of Functional Changes.Stem cells international · 2019Review
- Variation in primary and culture-expanded cells derived from connective tissue progenitors in human bone marrow space, bone trabecular surface and adipose tissue.Cytotherapy · 2018Article
- Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering.PloS one · 2017Article
- AAOS Research Symposium Updates and Consensus: Biologic Treatment of Orthopaedic Injuries.The Journal of the American Academy of Orthopaedic Surgeons · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Orthopedic implants including engineered bone tissue are commonly tested in sheep. To avoid rejection of heterologous or xenogeneic cells, autologous cells are preferably used, that is, ovine mesenchymal stem cells (oMSC). Unlike human MSC, ovine MSC are not well studied regarding isolation, expansion, and characterization. Here we investigated the impact of culture media composition on growth characteristics, differentiation, and surface antigen expression of oMSC. The culture media varied in fetal calf serum (FCS) content and in the addition of supplements and/or additional epidermal growth factor (EGF). We found that FCS strongly influenced oMSC proliferation and that specific combinations of supplemental factors (MCDB-201, ITS-plus, dexamethasone, and L-ascorbic acid) determined the expression of surface epitopes. We compared two published protocols for oMSC differentiation towards the osteogenic, adipogenic, and chondrogenic fate and found (i) considerable donor to donor variations, (ii) protocol-dependent variations, and (iii) variations resulting from the preculture medium composition. Our results indicate that the isolation and culture of oMSC in different growth media are highly variable regarding oMSC phenotype and behaviour. Furthermore, variations from donor to donor critically influence growth rate, surface marker expression, and differentiation.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.