ArticleTheScientificWorldJournal2013
A TaqMan real-time PCR assay for detection and quantification of Sporisorium scitamineum in sugarcane.
Article in TheScientificWorldJournal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Article
- Auxin dysregulation: a key early event in sugarcane susceptibility to Sporisorium scitamineum.BMC plant biology · 2026Article
- Pathogenesis, Host Resistance and Integrated Management of Sugarcane Smut Caused by Sporisorium scitamineum: A Comprehensive Review.Molecular plant pathology · 2025Review
- A Sugarcane Smut Fungus Effector Hijacks Plant Vacuolar Sorting Receptor-Mediated Trafficking to Evade Host Immune Detection.Plant, cell & environment · 2025Article
- Pathogenesis-Related 1 (PR1) Protein Family Genes Involved in Sugarcane Responses toInternational journal of molecular sciences · 2024Article
- Identification of Gene Modules and Hub Genes Associated withJournal of fungi (Basel, Switzerland) · 2022Article
- A sugarcane smut fungus effector simulates the host endogenous elicitor peptide to suppress plant immunity.The New phytologist · 2022Article
- Impact of Sporisorium scitamineum infection on the qualitative traits of commercial cultivars and advanced lines of sugarcane.PloS one · 2022Article
- Sugarcane Smut: Current Knowledge and the Way Forward for Management.Journal of fungi (Basel, Switzerland) · 2021Review
- Leaf Transcriptome Analysis of Broomcorn Millet Uncovers Key Genes and Pathways in Response toInternational journal of molecular sciences · 2021Article
- Comparative expression analysis of potential pathogenicity-associated genes of high- and low-virulent3 Biotech · 2021Article
- Proteomic Analysis of the Resistance Mechanisms in Sugarcane duringInternational journal of molecular sciences · 2019Article
- A dynamic degradome landscape on miRNAs and their predicted targets in sugarcane caused by Sporisorium scitamineum stress.BMC genomics · 2019Article
- Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Ustilago maydis.Scientific reports · 2017Article
- Plant jasmonate ZIM domain genes: shedding light on structure and expression patterns of JAZ gene family in sugarcane.BMC genomics · 2017Article
- Comparative proteomics reveals that central metabolism changes are associated with resistance against Sporisorium scitamineum in sugarcane.BMC genomics · 2016Article
- Development and application of a rapid and visual loop-mediated isothermal amplification for the detection of Sporisorium scitamineum in sugarcane.Scientific reports · 2016Article
- Early Selection for Smut Resistance in Sugarcane Using Pathogen Proliferation and Changes in Physiological and Biochemical Indices.Frontiers in plant science · 2016Article
- Biogeographical variation and population genetic structure of Sporisorium scitamineum in Mainland China: insights from ISSR and SP-SRAP markers.TheScientificWorldJournal · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sporisorium scitamineum is a fungal smut pathogen epidemic in sugarcane producing areas. Early detection and proper identification of the smut are an essential requirement in its management practice. In this study, we developed a TaqMan real-time PCR assay using specific primers (bEQ-F/bEQ-R) and a TaqMan probe (bEQ-P) which were designed based on the bE (b East mating type) gene (Genbank Accession no. U61290.1). This method was more sensitive (a detection limit of 10 ag pbE DNA and 0.8 ng sugarcane genomic DNA) than that of conventional PCR (10 fg and 100 ng, resp.). Reliability was demonstrated through the positive detection of samples collected from artificially inoculated sugarcane plantlets (FN40). This assay was capable of detecting the smut pathogen at the initial stage (12 h) of infection and suitable for inspection of sugarcane pathogen-free seed cane and seedlings. Furthermore, quantification of pathogen was verified in pathogen-challenged buds in different sugarcane genotypes, which suggested its feasibility for evaluation of smut resistance in different sugarcane genotypes. Taken together, this novel assay can be used as a diagnostic tool for sensitive, accurate, fast, and quantitative detection of the smut pathogen especially for asymptomatic seed cane or plants and evaluation of smut resistance of sugarcane genotypes.
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