Evidence map›Paper›PMID 24227746›Full record

ArticleBiotechnology journal2014

Microarray platform affords improved product analysis in mammalian cell growth studies.

Payel Datta, Luciana Meli, Lingyun Li, Nicole Migliore, Eugene Schaefer, Susan T Sharfstein, Jonathan S Dordick, Robert J Linhardt

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Cell-Free Protein Synthesis: A Promising Option for Future Drug Development.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2020
    Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Payel Datta *Department of Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY.
Luciana Meli *Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY.
Lingyun LiDepartment of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY.
Nicole MiglioreJanssen Biotech, Inc., Horsham, PA.
Eugene SchaeferJanssen Biotech, Inc., Horsham, PA.
Susan T SharfsteinCollege of Nanoscale Science and Engineering, University at Albany, Albany, NY.
Jonathan S DordickDepartment of Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY.
Robert J LinhardtDepartment of Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY.
Rensselaer Polytechnic Institute · USJanssen (United States) · USUniversity at Albany, State University of New York · US

Funding

SEQUENCING THE POLYSACCHARIDE COMPONENT OF PROTEOGLYCANSR01GM038060 · NIGMS · UNIVERSITY OF IOWA · PI LINHARDT, ROBERT J · 1988 to 2012
$2.8M
High-Throughput Platform for Identifying Stem Cell ToxicityR01ES020903 · NIEHS · RENSSELAER POLYTECHNIC INSTITUTE · PI DORDICK, JONATHAN S., SCHAFFER, DAVID V · 2012 to 2015
$2.0M
Metabolic Engineering of Chinese Hamster Ovary Cells for Production of HeparinR01GM090127 · NIGMS · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI SHARFSTEIN, SUSAN T · 2009 to 2011
$1.0M
NIEHS NIH HHS ES020903NIEHS NIH HHS R01 ES020903NIGMS NIH HHS GM090127NIGMS NIH HHS GM38060NIGMS NIH HHS R01 GM038060NIGMS NIH HHS R01 GM090127
6 · The paper itself

Abstract

High throughput (HT) platforms serve as a cost-efficient and rapid screening method for evaluating the effect of cell-culture conditions and screening of chemicals. We report the development of a HT cell-based microarray platform to assess the effect of culture conditions on Chinese hamster ovary (CHO) cells. Specifically, growth, transgene expression and metabolism of a GS/methionine sulphoximine (MSX) CHO cell line, which produces a therapeutic monoclonal antibody, was examined using a microarray system in conjunction with a conventional shake flask platform in a non-proprietary medium. The microarray system consists of 60-nL spots of cells encapsulated in alginate and separated in groups via an 8-well chamber system attached to the chip. Results show the non-proprietary medium developed allows cell growth, production, and normal glycosylation of recombinant antibody and metabolism of the recombinant CHO cells in both the microarray and shake flask platforms. In addition, 10.3 mM glutamate addition to the defined base medium results in lactate metabolism shift in the recombinant GS/MSX CHO cells in the shake flask platform. Ultimately, the results demonstrate that the HT microarray platform has the potential to be utilized for evaluating the impact of media additives on cellular processes, such as cell growth, metabolism, and productivity.

Indexed as

Cell ProliferationAnimalsAntibodies, MonoclonalCell Culture TechniquesCHO CellsCricetinaeCricetulusCulture MediaHigh-Throughput Screening AssaysMammalsRecombinant ProteinsTissue Array AnalysisAntibodies, MonoclonalCulture MediaRecombinant Proteins3D cell cultureCell-based microarrayCHO cellsHigh throughputMedia optimization

Identifiers

PMID24227746
PMCPMC4129516
OpenAlexW2154234393

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.