ArticleJournal of the American Society for Mass Spectrometry1993
Selective detection of phosphopeptides in complex mixtures by electrospray liquid chromatography/mass spectrometry.
Article in Journal of the American Society for Mass Spectrometry, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 242 citations in OpenAlex.
- Investigation and Development of the BODIPY-Embedded Isotopic Signature for Chemoproteomics Labeling and Targeted Profiling.Journal of the American Society for Mass Spectrometry · 2024Article
- Multimodal Tandem Mass Spectrometry Techniques for the Analysis of Phosphopeptides.Journal of the American Society for Mass Spectrometry · 2022Article
- Native Ambient Mass Spectrometry Enables Analysis of Intact Endogenous Protein Assemblies up to 145 kDa Directly from Tissue.Analytical chemistry · 2022Article
- Negative Ion Mode Collision-Induced Dissociation for Analysis of Protein Arginine Methylation.Journal of the American Society for Mass Spectrometry · 2019Article
- Lipidomic analysis of meibomian gland secretions from the tree shrew: Identification of candidate tear lipids critical for reducing evaporation.Chemistry and physics of lipids · 2019Article
- Discovery of the antibiotic phosacetamycin via a new mass spectrometry-based method for phosphonic acid detection.ACS chemical biology · 2013Article
- Functional phosphoproteomic mass spectrometry-based approaches.Clinical and translational medicine · 2012Article
- Relevant phosphoproteomic and mass spectrometry: approaches useful in clinical research.Clinical and translational medicine · 2012Article
- A Review of Tandem Mass Spectrometry Characterization of Adenosine Diphosphate-Ribosylated Peptides.International journal of mass spectrometry · 2012Article
- Identification of Phosphorylated Human Peptides by Accurate Mass Measurement Alone.International journal of mass spectrometry · 2011Article
- Effect of cysteic acid position on the negative ion fragmentation of proteolytic derived peptides.Journal of the American Society for Mass Spectrometry · 2011Article
- Comparative assessment of site assignments in CID and electron transfer dissociation spectra of phosphopeptides discloses limited relocation of phosphate groups.Molecular & cellular proteomics : MCP · 2010Article
- Sequence assignment of ADP-ribosylated peptides is facilitated as peptide length increases.Rapid communications in mass spectrometry : RCM · 2010Article
- Mass spectral characterization of organophosphate-labeled, tyrosine-containing peptides: characteristic mass fragments and a new binding motif for organophosphates.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2010Article
- Tandem mass spectrometry investigation of ADP-ribosylated kemptide.Journal of the American Society for Mass Spectrometry · 2009Article
- Mapping disulfide bonds in insulin with the Route 66 Method: selective cleavage of S-C bonds using alkali and alkaline earth metal enolate complexes.Journal of the American Society for Mass Spectrometry · 2009Article
- Atmospheric pressure thermal dissociation of phospho- and sulfopeptides.Journal of the American Society for Mass Spectrometry · 2008Article
- Phosphopeptide elution times in reversed-phase liquid chromatography.Journal of chromatography. A · 2007Article
- The combination of electron capture dissociation and fixed charge derivatization increases sequence coverage for O-glycosylated and O-phosphorylated peptides.Journal of the American Society for Mass Spectrometry · 2007Article
- Accurate mass measurements in proteomics.Chemical reviews · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A mass spectrometry-based method that does not involve the use of radiolabeling was developed for selective detection of phosphopeptides in complex mixtures. Mixtures of phosphorylated and nonphosphorylated peptides at the low picomole level are analyzed by negative ion electrospray liquid chromatography/mass spectrometry using C-18 packed fused-silica columns (≤320-μm i.d.). Peptides and phosphopeptides in the chromatographic eluant undergo collision-induced dissociation in the free-jet expansion region prior to the mass analyzing quadrupole. Using relatively high collisional excitation potentials, phospho|peptides containing phosphoserine, phosphothreonine, and phosphotyrosine fragment to yield diagnostic ions at m/z 63 and 79 corresponding to PO2 (-); and PO3 (-), respectively. Chromatographic peaks containing phosphopeptides are indicated where these diagnostic ions maximize. The highest sensitivity for phosphopeptide detection is obtained using selected-ion monitoring for m/z 63 and 79. Full-scan mass spectra that exhibit the diagnostic phosphopeptide fragment ions, together with pseudomolecular ions, may be obtained by stepping the collisional excitation potential from a high value during the portion of each scan in which the low-mass-to-charge ratio diagnostic marker ions are being detected to a lower value while the upper mass-to-charge ratio range is being scanned. Good sensitivity for phosphopeptide detection was achieved using standard trifluoroacetic acid containing mobile phases for reversed-phase high-performance liquid chromatography. Data illustrating the selectivity and sensitivity of the approach are presented for mixtures of peptides and phosphopeptides containing the three commonly phosphorylated amino acids.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.