ArticleScientific reports2013
A novel approach for separating bacteriophages from other bacteriophages using affinity chromatography and phage display.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Phage therapy and its role in cancer treatment and control.Folia microbiologica · 2025Review
- Monomeric streptavidin phage display allows efficient immobilization of bacteriophages on magnetic particles for the capture, separation, and detection of bacteria.Scientific reports · 2023Article
- Bacteriophages as Biotechnological Tools.Viruses · 2023Review
- Poly-Histidine-Tagged Protein Purification Using Immobilized Metal Affinity Chromatography (IMAC).Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Modern Techniques for the Isolation of Extracellular Vesicles and Viruses.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2020Review
- A High-Throughput Single-Clone Phage Fluorescence Microwell Immunoassay and Laser-Driven Clonal Retrieval System.Biomolecules · 2020Article
- Bacteriophages engineered to display foreign peptides may become short-circulating phages.Microbial biotechnology · 2019Article
- Bacteriophage T4 capsid as a nanocarrier for Peptide-N-Glycosidase F immobilization through self-assembly.Scientific reports · 2019Article
- Robust Approaches for the Production of Active Ingredient and Drug Product for Human Phage Therapy.Frontiers in microbiology · 2019Review
- Oral Application of T4 Phage Induces Weak Antibody Production in the Gut and in the Blood.Viruses · 2015Article
- Molecular and chemical engineering of bacteriophages for potential medical applications.Archivum immunologiae et therapiae experimentalis · 2015Review
- T4 bacteriophage as a phage display platform.Archives of microbiology · 2014Review
- Molecular imaging of T4 phage in mammalian tissues and cells.Bacteriophage · 2014Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Practical applications of bacteriophages in medicine and biotechnology induce a great need for technologies of phage purification. None of the popular methods offer solutions for separation of a phage from another similar phage. We used affinity chromatography combined with competitive phage display (i) to purify T4 bacteriophage from bacterial debris and (ii) to separate T4 from other contaminating bacteriophages. In 'competitive phage display' bacterial cells produced both wild types of the proteins (expression from the phage genome) and the protein fusions with affinity tags (expression from the expression vectors). Fusion proteins were competitively incorporated into the phage capsid. It allowed effective separation of T4 from a contaminating phage on standard affinity resins.
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