ArticleBMC genomics2013
The Gut Microbiotassay: a high-throughput qPCR approach combinable with next generation sequencing to study gut microbial diversity.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Impact of short-chain galactooligosaccharides on the gut microbiome of lactose-intolerant individuals.Proceedings of the National Academy of Sciences of the United States of America · 2017Trial
- Trial
- A dual-loop chemostat to investigate multi-species biofilms on implant surfaces under adjustable flow conditions.Frontiers in microbiology · 2026Article
- Identification of distinct stool metabolites in women with endometriosis for non-invasive diagnosis and potential for microbiota-based therapies.Med (New York, N.Y.) · 2025Article
- Assessment of salivary microbiota profile as a potential diagnostic tool for pediatric celiac disease.Scientific reports · 2024Article
- Updated Progress on Polysaccharides with Anti-Diabetic Effects through the Regulation of Gut Microbiota: Sources, Mechanisms, and Structure-Activity Relationships.Pharmaceuticals (Basel, Switzerland) · 2024Review
- Inter-year consistencies and discrepancies on intestinal microbiota for overwintering relict gulls: correlations with food composition and implications for environmental adaptation.Frontiers in microbiology · 2024Article
- Profiling of the fecal microbiota and circulating microRNA-16 in IBS subjects with Blastocystis infection : a case-control study.European journal of medical research · 2023Article
- Comparative analysis of the pig gut microbiome associated with the pig growth performance.Journal of animal science and technology · 2023Article
- Characterization of gut microbiota profile in Iranian patients with bipolar disorder compared to healthy controls.Frontiers in cellular and infection microbiology · 2023Article
- Intestinal microbiota changes pre- and post-fecal microbiota transplantation for treatment of recurrentFrontiers in microbiology · 2023Article
- Diet-driven microbial ecology underpins associations between cancer immunotherapy outcomes and the gut microbiome.Nature medicine · 2022Article
- ACancers · 2022Article
- Determination of the Optimal Level of Dietary Zinc for Newly Weaned Pigs: A Dose-Response Study.Animals : an open access journal from MDPI · 2022Article
- High-throughput qPCR and 16S rRNA gene amplicon sequencing as complementary methods for the investigation of the cheese microbiota.BMC microbiology · 2022Article
- Effects of Dietary Antimicrobial Growth Promoters on Performance Parameters and Abundance and Diversity of Broiler Chicken Gut Microbiome and Selection of Antibiotic Resistance Genes.Frontiers in microbiology · 2022Article
- The effects of preoperative intestinal dysbacteriosis on postoperative recovery in colorectal cancer surgery: a prospective cohort study.BMC gastroenterology · 2021Article
- Comparison of targeted metagenomics and IS-Pro methods for analysing the lung microbiome.BMC microbiology · 2021Article
- Characterization of the gut microbiota in patients with primary sclerosing cholangitis compared to inflammatory bowel disease and healthy controls.Molecular biology reports · 2021Article
- Modulation of the Gut Microbiota and Serum Biomarkers After Laparoscopic Sleeve Gastrectomy: a 1-Year Follow-Up Study.Obesity surgery · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe intestinal microbiota is a complex and diverse ecosystem that plays a significant role in maintaining the health and well-being of the mammalian host. During the last decade focus has increased on the importance of intestinal bacteria. Several molecular methods can be applied to describe the composition of the microbiota. This study used a new approach, the Gut Microbiotassay: an assembly of 24 primer sets targeting the main phyla and taxonomically related subgroups of the intestinal microbiota, to be used with the high-throughput qPCR chip 'Access Array 48.48', AA48.48, (Fluidigm®) followed by next generation sequencing. Primers were designed if necessary and all primer sets were screened against DNA extracted from pure cultures of 15 representative bacterial species. Subsequently the setup was tested on DNA extracted from small and large intestinal content from piglets with and without diarrhoea. The PCR amplicons from the 2304 reaction chambers were harvested from the AA48.48, purified, and sequenced using 454-technology.
resultsThe Gut Microbiotassay was able to detect significant differences in the quantity and composition of the microbiota according to gut sections and diarrhoeic status. 454-sequencing confirmed the specificity of the primer sets. Diarrhoea was associated with a reduced number of members from the genus Streptococcus, and in particular S. alactolyticus.
conclusionThe Gut Microbiotassay provides fast and affordable high-throughput quantification of the bacterial composition in many samples and enables further descriptive taxonomic information if combined with 454-sequencing.
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