ArticleTissue engineering. Part A2014
Mesenchymal stem cell-conditioned medium enhances osteogenic and chondrogenic differentiation of human embryonic stem cells and human induced pluripotent stem cells by mesodermal lineage induction.
Article in Tissue engineering. Part A, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Induced pluripotent stem cells as a new getaway for bone tissue engineering: A systematic review.Cell proliferation · 2017Pooled it
- Research advances in mesenchymal stem cells and related therapies for rotator cuff tendon-to-bone healing.Frontiers in bioengineering and biotechnology · 2025Review
- Induced Pluripotent Stem Cells in Dental and Nondental Tissue Regeneration: A Review of an Unexploited Potential.Stem cells international · 2020Review
- LncRNA HOTAIRM1 promotes osteogenesis by controlling JNK/AP-1 signalling-mediated RUNX2 expression.Journal of cellular and molecular medicine · 2019Article
- Biofunctionalized Scaffold in Bone Tissue Repair.International journal of molecular sciences · 2018Article
- Low Osteogenic Yield in Human Pluripotent Stem Cells Associates with Differential Neural Crest Promoter Methylation.Stem cells (Dayton, Ohio) · 2018Article
- A cancer stem cell model as the point of origin of cancer-associated fibroblasts in tumor microenvironment.Scientific reports · 2017Article
- Proteomic Profiling of Neuroblastoma Cells Adhesion on Hyaluronic Acid-Based Surface for Neural Tissue Engineering.BioMed research international · 2016Article
- Microencapsulated equine mesenchymal stromal cells promote cutaneous wound healing in vitro.Stem cell research & therapy · 2015Article
- Functional maintenance of differentiated embryoid bodies in microfluidic systems: a platform for personalized medicine.Stem cells translational medicine · 2015Article
- Challenges in engineering osteochondral tissue grafts with hierarchical structures.Expert opinion on biological therapy · 2015Review
- Efficient derivation of osteoprogenitor cells from induced pluripotent stem cells for bone regeneration.International orthopaedics · 2014Article
Corrections and comments
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Authors and funding
8 authors.
Funding
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Abstract
Human mesenchymal stem cells (hMSCs) have the ability to differentiate into mesenchymal lineages. In this study, we hypothesized that treatment of embryoid bodies (EBs) composed of either human embryonic stem cells (hESCs) or human induced pluripotent stem cells (hiPSCs) with a hMSC-conditioned medium (CM) can stimulate the induction of the mesodermal lineage and subsequent differentiation toward the osteogenic and chondrogenic lineage. Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) analysis indicated that the hMSC-CM treatment increased gene expression related to the mesodermal lineage and decreased gene expression related to the endodermal and ectodermal lineage in EBs. Fourteen days after culturing the mesodermal lineage-induced EBs in the osteogenic or chondrogenic differentiation medium, we observed enhanced osteogenic and chondrogenic differentiation compared with untreated EBs, as evaluated using qRT-PCR, cytochemistry, immunocytochemistry, and flow cytometry. This method may be useful for enhancing the osteogenic or chondrogenic differentiation of hESCs or hiPSCs.
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