ArticleFrontiers in oncology2013
Direct cell lysis for single-cell gene expression profiling.
Article in Frontiers in oncology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 58 citations in OpenAlex.
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- Low-toxic and organic solvent-free isolation of RNA.PloS one · 2026Article
- Injectable Piezoelectric Hydrogel for Vital Pulp Therapy.Journal of functional biomaterials · 2025Article
- Technical Considerations of Pharmacokinetic Assays for LNP-mRNA Drug Products by RT-qPCR.The AAPS journal · 2025Article
- Article
- A traveling surface acoustic wave-based micropiezoactuator: A tool for additive- and label-free cell lysis.Biomicrofluidics · 2024Article
- Highly Efficient On-Chip Photothermal Cell Lysis for Nucleic Acid Extraction Using Localized Plasmonic Heating of Strongly Absorbing Au Nanoislands.ACS applied materials & interfaces · 2023Article
- Article
- An Optimized Direct Lysis Gene Expression Microplate Assay and Applications for Disease, Differentiation, and Pharmacological Cell-Based Studies.Biosensors · 2022Article
- Article
- Tutorial: Guidelines for Single-Cell RT-qPCR.Cells · 2021Review
- Performance of a scalable RNA extraction-free transcriptome profiling method for adherent cultured human cells.Scientific reports · 2021Article
- Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-630.Scientific reports · 2021Article
- High-throughput full-length single-cell RNA-seq automation.Nature protocols · 2021Article
- A simple RNA preparation method for SARS-CoV-2 detection by RT-qPCR.Scientific reports · 2020Article
- Facile Fabrication of Thin-Bottom Round-Well Plates Using the Deformation of PDMS Molds and Their Application for Single-Cell PCR.Micromachines · 2020Article
- One step DNA amplification of mammalian cells in picoliter microwell arrays.RSC advances · 2019Article
- Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons.International journal of molecular sciences · 2018Article
- Unravelling the biological secrets of microchimerism by single-cell analysis.Briefings in functional genomics · 2018Article
- Identification of inhibitors regulating cell proliferation and FUS-DDIT3 expression in myxoid liposarcoma using combined DNA, mRNA, and protein analyses.Laboratory investigation; a journal of technical methods and pathology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interest to analyze single and few cell samples is rapidly increasing. Numerous extraction protocols to purify nucleic acids are available, but most of them compromise severely on yield to remove contaminants and are therefore not suitable for the analysis of samples containing small numbers of transcripts only. Here, we evaluate 17 direct cell lysis protocols for transcript yield and compatibility with downstream reverse transcription quantitative real-time PCR. Four endogenously expressed genes are assayed together with RNA and DNA spikes in the samples. We found bovine serum albumin (BSA) to be the best lysis agent, resulting in efficient cell lysis, high RNA stability, and enhanced reverse transcription efficiency. Furthermore, we found direct cell lysis with BSA superior to standard column based extraction methods, when analyzing from 1 up to 512 mammalian cells. In conclusion, direct cell lysis protocols based on BSA can be applied with most cell collection methods and are compatible with most analytical workflows to analyze single-cells as well as samples composed of small numbers of cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.