ArticlePloS one2013
Functional characterization of a wheat NHX antiporter gene TaNHX2 that encodes a K(+)/H(+) exchanger.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 49 citations in OpenAlex.
- Salinity signaling networks in wheat: crosstalk among CaPlant signaling & behavior · 2026Review
- The potassium transporter TaNHX2 interacts with TaGAD1 to promote drought tolerance via modulating stomatal aperture in wheat.Science advances · 2024Article
- Yeast Heterologous Expression Systems for the Study of Plant Membrane Proteins.International journal of molecular sciences · 2023Review
- Salinity stress tolerance and omics approaches: revisiting the progress and achievements in major cereal crops.Heredity · 2022Review
- Genome-Wide Identification and Functional Characterization of the Chloride Channel TaCLC Gene Family in Wheat (Frontiers in genetics · 2022Article
- Deciphering Reserve Mobilization, Antioxidant Potential, and Expression Analysis of Starch Synthesis in Sorghum Seedlings under Salt Stress.Plants (Basel, Switzerland) · 2021Article
- Genome-Wide Transcriptome Profiling, Characterization, and Functional Identification ofAntioxidants (Basel, Switzerland) · 2021Article
- Gene structure of three kinds of vacuolar-type Na+/H+ antiporters including TaNHX2 transcribed in bread wheat.Genetics and molecular biology · 2021Article
- Genome-Wide Identification of theInternational journal of molecular sciences · 2020Article
- A novel tonoplast NaPlant cell reports · 2020Article
- Ion Binding and Selectivity of the NaThe journal of physical chemistry. B · 2020Article
- Genome-Wide Identification and Analysis of HAK/KUP/KT Potassium Transporters Gene Family in Wheat (International journal of molecular sciences · 2018Article
- Mapping QTLs conferring salt tolerance and micronutrient concentrations at seedling stagein wheat.Scientific reports · 2017Article
- The Role of NaFrontiers in physiology · 2017Review
- The wheat NHX antiporter gene TaNHX2 confers salt tolerance in transgenic alfalfa by increasing the retention capacity of intracellular potassium.Plant molecular biology · 2015Article
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8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The subcellular localization of a wheat NHX antiporter, TaNHX2, was studied in Arabidopsis protoplasts, and its function was evaluated using Saccharomyces cerevisiae as a heterologous expression system. Fluorescence patterns of TaNHX2-GFP fusion protein in Arabidopsis cells indicated that TaNHX2 localized at endomembranes. TaNHX2 has significant sequence homology to NHX sodium exchangers from Arabidopsis, is abundant in roots and leaves and is induced by salt or dehydration treatments. Western blot analysis showed that TaNHX2 could be expressed in transgenic yeast cells. Expressed TaNHX2 protein suppressed the salt sensitivity of a yeast mutant strain by increasing its K(+) content when exposed to salt stress. TaNHX2 also increased the tolerance of the strain to potassium stress. However, the expression of TaNHX2 did not affect the sodium concentration in transgenic cells. Western blot analysis for tonoplast proteins indicated that the TaNHX2 protein localized at the tonoplast of transgenic yeast cells. The tonoplast vesicles from transgenic yeast cells displayed enhanced K(+)/H(+) exchange activity but very little Na(+/)H(+) exchange compared with controls transformed with the empty vector; Na(+)/H(+) exchange was not detected with concentrations of less than 37.5 mM Na(+) in the reaction medium. Our data suggest that TaNHX2 is a endomembrane-bound protein and may primarily function as a K(+)/H(+) antiporter, which is involved in cellular pH regulation and potassium nutrition under normal conditions. Under saline conditions, the protein mediates resistance to salt stress through the intracellular compartmentalization of potassium to regulate cellular pH and K(+) homeostasis.
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