ArticlePloS one2013
Identification and characterization of the transcriptional regulator ChrB in the chromate resistance determinant of Ochrobactrum tritici 5bvl1.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Novel polymyxin resistance gene family mcr-12 from environmental Pigmentiphaga litoralis.Nature communications · 2026Article
- Operonic architecture of bacterial metal response: envelope constraints, evolutionary mobility, and bioremediation design rules.World journal of microbiology & biotechnology · 2026Review
- Physicochemistry and comparative metagenomics of a tropical estuary persistently inundated with anthropogenic pollutants.Folia microbiologica · 2025Article
- An animal charcoal contaminated cottage industry soil highlighted by halophilic archaea dominance and decimation of bacteria.World journal of microbiology & biotechnology · 2024Article
- A PadR family transcriptional repressor regulates the transcription of chromate efflux transporter in Enterobacter sp. Z1.Journal of microbiology (Seoul, Korea) · 2024Article
- Adsorption of HgBMC biotechnology · 2024Article
- Chromium contamination accentuates changes in the microbiome and heavy metal resistome of a tropical agricultural soil.World journal of microbiology & biotechnology · 2023Article
- Rapid and Finely-Tuned Expression for Deployable Sensing Applications.Advances in biochemical engineering/biotechnology · 2023Article
- Plant growth-promoting effect and genomic analysis of the P. putida LWPZF isolated from C. japonicum rhizosphere.AMB Express · 2022Article
- Whole-genome sequencing and comparative analysis of heavy metals tolerantAIMS microbiology · 2022Article
- Removal of Chromium (VI) byJournal of microbiology and biotechnology · 2020Article
- Two plant-hosted whole-cell bacterial biosensors for detection of bioavailable Cr(VI).World journal of microbiology & biotechnology · 2019Article
- Chromate tolerance and removal of bacterial strains isolated from uncontaminated and chromium-polluted environments.World journal of microbiology & biotechnology · 2019Article
- ChrASO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction.PloS one · 2017Article
- Prevalence, Host Range, and Comparative Genomic Analysis of TemperateFrontiers in microbiology · 2017Article
- Two superoxide dismutases from TnOtchr are involved in detoxification of reactive oxygen species induced by chromate.BMC microbiology · 2016Article
- Characterization and structure prediction of partial length protein sequences of pcoA, pcoR and chrB genes from heavy metal resistant bacteria from the Klip River, South Africa.International journal of molecular sciences · 2015Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ochrobactrum tritici 5bvl1 is able to resist to high concentrations of chromate through the expression of an inducible chromate-resistant determinant, found in a mobile element (TnOtChr), which carries the genes, chrB, chrA, chrC and chrF. The regulation of chr operon present in TnOtChr, which is controlled by a transcriptional regulator, ChrB, was characterized in the current work. Fusions of chr promoter, or chr promoter and chrB gene, upstream of a gfp reporter gene, identified the most probable promoter sequence within the tnpR-chrB intergenic region. This region contains an AT-rich imperfect inverted repeat sequence, which overlaps a part of the -10 sequence. The results of the in vitro DNA-binding assays with purified ChrB (His- or no-tagged) showed that the protein binds directly to the chr promoter region. In order to identify the ChrB functional domain for sensing chromate stress and for DNA-binding, site-directed mutagenesis of ChrB was performed. Among several single amino acid mutants, three mutants (R180; R187 and H229) prevented chromate induction without any modification to the protein's stability. Interestingly, two ChrB mutants (R18 and R23) were constitutively active, regardless of chromate stress conditions, indicating that the residues most probably belong to the protein-DNA binding site. As such, the ChrB was classified as a transcriptional regulator that recognizes a specific DNA sequence, regulating the expression of a chromate resistance determinant.
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