ArticlePloS one2013
Incorporation of phosphatase inhibitor in culture prompts growth initiation of isolated non-growing oocytes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- The role of leptin in regulation of the soluble amyloid precursor protein α (sAPPα) levels in lung cancer cell media.Scientific reports · 2024Article
- Preservation of fertility in female and male prepubertal patients diagnosed with cancer.Journal of assisted reproduction and genetics · 2023Review
- Article
- A way for in vitro/ex vivo egg production in mammals.The Journal of reproduction and development · 2019Review
- Effects of insulin-like growth factor-1 on the in vitro maturation of canine oocytes.The Journal of reproduction and development · 2018Article
- PTEN Inhibition in Human Disease Therapy.Molecules (Basel, Switzerland) · 2018Review
- Developmental competence of oocytes grown in vitro: Has it peaked already?The Journal of reproduction and development · 2016Article
- MicroRNAs in ovarian function and disorders.Journal of ovarian research · 2015Review
- Spontaneous γH2AX Foci in Human Solid Tumor-Derived Cell Lines in Relation to p21WAF1 and WIP1 Expression.International journal of molecular sciences · 2015Article
- Cytokine (IL16) and tyrphostin actions on ovarian primordial follicle development.Reproduction (Cambridge, England) · 2014Article
- Inhibition of phosphatase and tensin homologue (PTEN) in human ovary in vitro results in increased activation of primordial follicles but compromises development of growing follicles.Molecular human reproduction · 2014Article
- Short Term Culture of Vitrified Human Ovarian Cortical Tissue to Assess the Cryopreservation Outcome: Molecular and Morphological Analysis.Journal of reproduction & infertilityArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro folliculogenesis of primordial and early preantral follicles is necessary for increment of reproductive efficiency in domestic animals, humans and endangered species. Recent study in phosphatase and tensin homolog (Pten) -knockout mice has revealed that this phosphatase acts as an inhibitory factor in follicle activation of primordial pool with the resultant inhibition of oocyte growth. To test in vitro effect of a phosphatase inhibitor on growth initiation of isolated non-growing oocytes in neonatal ovaries, we applied a specific inhibitor (bpV (HOpic)) for PTEN in culturing system. Non-growing oocytes isolated from the ovaries of newborn BDF1 (C57BL/6 × DBA/2) pups were divided to four culture groups. Five days after culture, the oocytes in 14 μmol/l bpV only, 14 μmol/l bpV plus 100 ng/ml Kit Ligand (KL), and 100 ng/ml KL groups showed significantly (P<0.05) growth (19.3 ± 0.55, 25.8 ± 0.53 and 21.6 ± 0.29 μm, respectively) compared with that of the control (no additive) (16.9 ± 0.53 μm). In addition, western blotting in those groups showed enhanced expression of phosphorylated Akt. In conclusion, we clearly demonstrate that isolated non-growing oocytes develop in phosphatase inhibitor, especially to PTEN, incorporated culturing system, and show first as we know that oocytes with zona Pellucidae can be obtained in vitro from isolated non-growing oocytes.
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