ArticleExperimental and therapeutic medicine2013
Human amniotic epithelial cell feeder layers maintain iPS cell pluripotency by inhibiting endogenous DNA methyltransferase 1.
Article in Experimental and therapeutic medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 12 citations in OpenAlex.
- Androgen Deprivation Induces Transcriptional Reprogramming in Prostate Cancer Cells to Develop Stem Cell-Like Characteristics.International journal of molecular sciences · 2020Article
- Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization.Molecular biology of the cell · 2017Article
- Hair follicle-derived mesenchymal cells support undifferentiated growth of embryonic stem cells.Experimental and therapeutic medicine · 2017Article
- Lacrimal Gland Repair Using Progenitor Cells.Stem cells translational medicine · 2017Article
- First steps to define murine amniotic fluid stem cell microenvironment.Scientific reports · 2016Article
- Small molecule compounds alleviate anisomycin-induced oxidative stress injury in SH-SY5Y cells via downregulation of p66shc and Aβ1-42 expression.Experimental and therapeutic medicine · 2016Article
- Gestational stage affects amniotic epithelial cells phenotype, methylation status, immunomodulatory and stemness properties.Stem cell reviews and reports · 2014Article
- Human amniotic fluid stem cells support undifferentiated propagation and pluripotency of human embryonic stem cell without b-FGF in a density dependent manner.International journal of clinical and experimental pathology · 2014Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maintaining induced pluripotent stem (iPS) cells in an undifferentiated, self-renewing state during long-term cultivation is, at present, a major challenge. We previously showed that human amniotic epithelial cells (HuAECs) were able to provide a good source of feeder cells for mouse and human embryonic or spermatogonial stem cells; however, the epigenetic mechanisms have not been elucidated. In the present study, mouse embryonic fibroblasts (MEFs) and HuAECs were compared as feeder layers for the long-term culture of human iPS cells. The HuAEC feeders allowed human iPS cells to maintain a high level of alkaline phosphatase (AP) activity and to express key stem cell markers during long-term subculture whereas the MEF feeders did not,. Moreover, the HuAEC feeders significantly affected the cell cycle regulation of the iPS cells, maintaining them in the resting stage and the early stage of DNA synthesis (G0/G1 stage). Furthermore, the CpG islands of the
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