ArticlePloS one2013
Utility of host delivered RNAi of two FMRF amide like peptides, flp-14 and flp-18, for the management of root knot nematode, Meloidogyne incognita.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Chemosensory Pathways Involved in the Perception of Tomato-Derived Flavonoids and Alkaloids inPathogens (Basel, Switzerland) · 2026Article
- Toward sustainable control of phyto-nematodes: integrating lessons from crops to advance genetic modification in tomato.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Review
- Biocontrol of Root-Knot Nematodes via siRNA-Loaded Extracellular Vesicles From a Nematophagous Fungus Arthrobotrys oligospora.Microbial biotechnology · 2025Article
- Article
- GPCR Sense Communication Among Interaction Nematodes with Other Organisms.International journal of molecular sciences · 2025Review
- Meloidogyne incognita genes involved in the repellent behavior in response to ascr#9.Scientific reports · 2024Article
- Biochemical and nanotechnological approaches to combat phytoparasitic nematodes.Plant biotechnology journal · 2024Review
- Host-induced RNA interference targeting the neuromotor gene FMRFamide-like peptide-14 (Mi-flp14) perturbs Meloidogyne incognita parasitic success in eggplant.Plant cell reports · 2024Article
- Unraveling the Roles of Neuropeptides in the Chemosensation of the Root-Knot NematodeInternational journal of molecular sciences · 2024Article
- Induced knockdown ofHeliyon · 2024Article
- Molecular and functional characterization of chemosensory genes from the root-knot nematode Meloidogyne graminicola.BMC genomics · 2023Article
- Transgenic Improvement for Biotic Resistance of Crops.International journal of molecular sciences · 2022Review
- Exploiting Plant-Phytonematode Interactions to Upgrade Safe and Effective Nematode Control.Life (Basel, Switzerland) · 2022Review
- Understanding Molecular Plant-Nematode Interactions to Develop Alternative Approaches for Nematode Control.Plants (Basel, Switzerland) · 2022Review
- Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress.BMC plant biology · 2021Article
- Host-mediated RNAi for simultaneous silencing of different functional groups of genes in Meloidogyne incognita using fusion cassettes in Nicotiana tabacum.Plant cell reports · 2021Article
- Article
- RNA interference and crop protection against biotic stresses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Article
- Cyst nematode bio-communication with plants: implications for novel management approaches.Pest management science · 2021Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Root knot nematode, Meloidogyne incognita, is an obligate sedentary endoparasite that infects a large number of crop species and causes substantial yield losses. Non-chemical based control strategies for these nematodes are gaining importance. In the present study, we have demonstrated the significance of two FMRFamide like peptide genes (flp-14 and flp-18) for infection and development of resistance to M. incognita through host-derived RNAi. The study demonstrated both in vitro and in planta validation of RNAi-induced silencing of the two genes cloned from J2 stage of M. incognita. In vitro silencing of both the genes interfered with nematode migration towards the host roots and subsequent invasion into the roots. Transgenic tobacco lines were developed with RNAi constructs of flp-14 and flp-18 and evaluated against M. incognita. The transformed plants did not show any visible phenotypic variations suggesting the absence of any off-target effects. Bioefficacy studies with deliberate challenging of M. incognita resulted in 50-80% reduction in infection and multiplication confirming the silencing effect. We have provided evidence for in vitro and in planta silencing of the genes by expression analysis using qRT-PCR. Thus the identified genes and the strategy can be used as a potential tool for the control of M. incognita. This is the first ever report that has revealed the utility of host delivered RNAi of flps to control M. incognita. The strategy can also be extended to other crops and nematodes.
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