ArticlePloS one2013
Identification of estrogen target genes during zebrafish embryonic development through transcriptomic analysis.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 3 of them syntheses that pooled it.
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Who cites it
41 citing papers in PubMed, 3 syntheses or guidelines pooled it, 118 citations in OpenAlex.
- A systematic review on the evaluation of endocrine-disrupting chemicals as potential neurotoxins during zebrafish development.Frontiers in endocrinology · 2026Pooled it
- Sex-specific differences in zebrafish brains.Biology of sex differences · 2022Pooled it
- The Transcriptome of the Zebrafish Embryo After Chemical Exposure: A Meta-Analysis.Toxicological sciences : an official journal of the Society of Toxicology · 2017Pooled it
- Disruption of estrogen signaling by developmental exposure to BPA and TBT causes long term functional deficits in zebrafish retina.Frontiers in pharmacology · 2026Review
- Developmental Delay and Male-Biased Sex Ratio inGenes · 2024Article
- Comparing Transcriptomic Points of Departure to Apical Effect Concentrations For Larval Fathead Minnow Exposed to Chemicals with Four Different Modes Of Action.Archives of environmental contamination and toxicology · 2024Article
- Clomiphene Citrate and Enclomiphene Hydrochloride Exposure Is Associated With Interfrontal Suture Fusion in Zebrafish.Plastic surgery (Oakville, Ont.) · 2023Article
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- Zebrafish as an emerging model to study estrogen receptors in neural development.Frontiers in endocrinology · 2023Review
- Estrogens revert neutrophil hyperplasia by inhibiting Hif1α-cMyb pathway in zebrafish myelodysplastic syndromes models.Cell death discovery · 2022Article
- Soy-Derived Equol Induces Antioxidant Activity in Zebrafish in an Nrf2-Independent Manner.International journal of molecular sciences · 2022Article
- Protein-Protein Interaction (PPI) Network of Zebrafish Oestrogen Receptors: A Bioinformatics Workflow.Life (Basel, Switzerland) · 2022Review
- Estrogen promotes innate immune evasion of Candida albicans through inactivation of the alternative complement system.Cell reports · 2022Article
- The Role of Estrogen and Thyroid Hormones in Zebrafish Visual System Function.Frontiers in pharmacology · 2022Review
- Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The phenotypic and transcriptomic effects of developmental exposure to nanomolar levels of estrone and bisphenol A in zebrafish.The Science of the total environment · 2021Article
- A fish with no sex: gonadal and adrenal functions partition between zebrafish NR5A1 co-orthologs.Genetics · 2021Article
- Cisplatin-induced hair cell loss in zebrafish neuromasts is accompanied by protein nitration and Lmo4 degradation.Toxicology and applied pharmacology · 2021Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Estrogen signaling is important for vertebrate embryonic development. Here we have used zebrafish (Danio rerio) as a vertebrate model to analyze estrogen signaling during development. Zebrafish embryos were exposed to 1 µM 17β-estradiol (E2) or vehicle from 3 hours to 4 days post fertilization (dpf), harvested at 1, 2, 3 and 4 dpf, and subjected to RNA extraction for transcriptome analysis using microarrays. Differentially expressed genes by E2-treatment were analyzed with hierarchical clustering followed by biological process and tissue enrichment analysis. Markedly distinct sets of genes were up and down-regulated by E2 at the four different time points. Among these genes, only the well-known estrogenic marker vtg1 was co-regulated at all time points. Despite this, the biological functional categories targeted by E2 were relatively similar throughout zebrafish development. According to knowledge-based tissue enrichment, estrogen responsive genes were clustered mainly in the liver, pancreas and brain. This was in line with the developmental dynamics of estrogen-target tissues that were visualized using transgenic zebrafish containing estrogen responsive elements driving the expression of GFP (Tg(5xERE:GFP)). Finally, the identified embryonic estrogen-responsive genes were compared to already published estrogen-responsive genes identified in male adult zebrafish (Gene Expression Omnibus database). The expressions of a few genes were co-regulated by E2 in both embryonic and adult zebrafish. These could potentially be used as estrogenic biomarkers for exposure to estrogens or estrogenic endocrine disruptors in zebrafish. In conclusion, our data suggests that estrogen effects on early embryonic zebrafish development are stage- and tissue- specific.
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