ArticleTranslational vision science & technology2013
Optimization of Ex Vivo Expansion of Limbal Epithelial Progenitors by Maintaining Native Niche Cells on Denuded Amniotic Membrane.
Article in Translational vision science & technology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Article
- A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction.International journal of molecular sciences · 2021Article
- KIT ligand produced by limbal niche cells under control of SOX10 maintains limbal epithelial stem cell survival by activating the KIT/AKT signalling pathway.Journal of cellular and molecular medicine · 2020Article
- Limbal niche cells are a potent resource of adult mesenchymal progenitors.Journal of cellular and molecular medicine · 2018Review
- Preservation of epithelial progenitor cells from collagenase-digested oral mucosa during ex vivo cultivation.Scientific reports · 2016Article
- A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells.Stem cell research · 2016Article
- The Limbal Epithelial Progenitors in the Limbal Niche Environment.International journal of medical sciences · 2016Review
- Human corneal stromal stem cells support limbal epithelial cells cultured on RAFT tissue equivalents.Scientific reports · 2015Article
- microRNA-103/107 Family Regulates Multiple Epithelial Stem Cell Characteristics.Stem cells (Dayton, Ohio) · 2015Article
- Human limbal mesenchymal cells support the growth of human corneal epithelial stem/progenitor cells.Investigative ophthalmology & visual science · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
purposeTransplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on epithelially denuded amniotic membrane (dAM) in supplemented hormonal epithelial medium (SHEM) is an alternative solution for treating corneal blindness due to limbal stem cell (SC) deficiency. Because the phenotype of limbal niche cells (NCs) is preserved better in serum-free modified embryonic stem cell (ESC) medium (MESCM) than SHEM, we question whether the aforementioned expansion protocol can be further optimized by maintaining limbal NCs using MESCM.
methodsCollagenase-isolated limbal clusters were cultured on dAM in SHEM or MESCM for 8 to 10 days. Epithelial outgrowth sheets removed by dispase were subjected to real-time quantitative polymerase chain reaction (qPCR) and immunostaining for expression of corneal epithelial markers (p63
resultsEpithelial outgrowth in SHEM showed a significant loss of corneal SC and ESC markers when compared with freshly collagenase-isolated limbal clusters. Although the epithelial outgrowth was slower in MESCM, epithelial cell size was consistently smaller than that found in SHEM. Furthermore, MESCM maintained a significantly higher percentage of PCK-/ Vim+ cells and exhibited a significant upregulation of NC markers and corneal epithelial SC markers (K15, Bmi-1, and Msi-1) than SHEM. Furthermore, the number of purported holoclones was significantly promoted in MESCM than SHEM.
conclusionThese data collectively suggest that MESCM can be used to replace SHEM to further promote expansion of LEPC by maintaining limbal native NCs. TRANSLATIONAL RELEVANCE: Effective ex vivo expansion of limbal epithelial SC is a first and important step toward the success of treating corneal blindness caused by limbal stem cell deficiency and paves the way for future applications in regenerative medicine.
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