ArticleProceedings of the National Academy of Sciences of the United States of America2013
Antibody biomarker discovery through in vitro directed evolution of consensus recognition epitopes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- An electrochemical proximity assay (ECPA) for antibody detection incorporating flexible spacers for improved performance.Analytical and bioanalytical chemistry · 2024Article
- Liquid biopsy in T-cell lymphoma: biomarker detection techniques and clinical application.Molecular cancer · 2024Review
- Production of monoclonal antibody of heat-labile toxin A subunit to identify enterotoxigenicFrontiers in immunology · 2023Article
- Single-cell approaches to dissect adaptive immune responses involved in autoimmunity: the case of celiac disease.Mucosal immunology · 2022Review
- A MultipleBrain sciences · 2020Article
- Recent Developments of Electrochemical and Optical Biosensors for Antibody Detection.International journal of molecular sciences · 2019Review
- Identification of Peptide Mimotope Ligands for Natalizumab.Scientific reports · 2018Article
- The roles of MHC class II genes and post-translational modification in celiac disease.Immunogenetics · 2017Review
- Identification of disease-specific motifs in the antibody specificity repertoire via next-generation sequencing.Scientific reports · 2016Article
- Transglutaminase is a Critical Link Between Inflammation and Hypertension.Journal of the American Heart Association · 2016Article
- Gluten-specific antibodies of celiac disease gut plasma cells recognize long proteolytic fragments that typically harbor T-cell epitopes.Scientific reports · 2016Article
- Determination of B-Cell Epitopes in Patients with Celiac Disease: Peptide Microarrays.PloS one · 2016Article
- Serology in the 21st century: the molecular-level analysis of the serum antibody repertoire.Current opinion in immunology · 2015Review
- Epitope identification from fixed-complexity random-sequence peptide microarrays.Molecular & cellular proteomics : MCP · 2015Article
- Restricted VH/VL usage and limited mutations in gluten-specific IgA of coeliac disease lesion plasma cells.Nature communications · 2014Article
- Persistence of elevated deamidated gliadin peptide antibodies on a gluten-free diet indicates nonresponsive coeliac disease.Alimentary pharmacology & therapeutics · 2014Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
To enable discovery of serum antibodies indicative of disease and simultaneously develop reagents suitable for diagnosis, in vitro directed evolution was applied to identify consensus peptides recognized by patients' serum antibodies. Bacterial cell-displayed peptide libraries were quantitatively screened for binders to serum antibodies from patients with celiac disease (CD), using cell-sorting instrumentation to identify two distinct consensus epitope families specific to CD patients (PEQ and (E)/DxFV(Y)/FQ). Evolution of the (E)/DxFV(Y)/FQ consensus epitope identified a celiac-specific epitope, distinct from the two CD hallmark antigens tissue transglutaminase-2 and deamidated gliadin, exhibiting 71% sensitivity and 99% specificity (n = 231). Expansion of the first-generation PEQ consensus epitope via in vitro evolution yielded octapeptides QPEQAFPE and PFPEQxFP that identified ω- and γ-gliadins, and their deamidated forms, as immunodominant B-cell epitopes in wheat and related cereal proteins. The evolved octapeptides, but not first-generation peptides, discriminated one-way blinded CD and non-CD sera (n = 78) with exceptional accuracy, yielding 100% sensitivity and 98% specificity. Because this method, termed antibody diagnostics via evolution of peptides, does not require prior knowledge of pathobiology, it may be broadly useful for de novo discovery of antibody biomarkers and reagents for their detection.
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