ArticleInvestigative ophthalmology & visual science2014
Enzymatic degradation identifies components responsible for the structural properties of the vitreous body.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 57 citations in OpenAlex.
- Canonical PKCα-immunoreactive rod bipolar cells are present in nocturnal snakes but not in diurnal snakes.Scientific reports · 2026Article
- Preparing Porcine Eyes for Confocal Reflectance Microscopy to Visualize the Vitreous Collagen Fiber Network.Journal of visualized experiments : JoVE · 2025Article
- Replacing the vitreous body with hydrogels: Rationale and strategies.Progress in retinal and eye research · 2025Review
- High-fat diet alters retinal lipid composition and gene expression networks in mice.BMC biology · 2025Article
- Viscoelastic, Optical, and Surgical Properties of Vitreous Body Replacement Hydrogels After Aging Compared to Porcine Vitreous Bodies And Silicone Oils.Translational vision science & technology · 2024Article
- Forward Light Scattering of the Vitreous Gel After Enzymatic Aging: An In Vitro Model to Study Vitreous Opacification.Investigative ophthalmology & visual science · 2024Article
- Forward Light Scattering of First to Third Generation Vitreous Body Replacement Hydrogels after Surgical Application Compared to Conventional Silicone Oils and Vitreous Body.Gels (Basel, Switzerland) · 2023Article
- An Inverse Method to Determine Mechanical Parameters of Porcine Vitreous Bodies Based on the Indentation Test.Bioengineering (Basel, Switzerland) · 2023Article
- Confocal reflectance microscopy for mapping collagen fiber organization in the vitreous gel of the eye.Biomedical optics express · 2023Article
- Changes in the vitreous body after experimental vitreous hemorrhage in rabbit: An interdisciplinary study.PloS one · 2023Article
- Structure and mechanics of the vitreoretinal interface.Journal of the mechanical behavior of biomedical materials · 2022Review
- Dynamic Pressure Measurements During Vitrectomy in a Model of the Eye.Translational vision science & technology · 2022Article
- Laser-induced nanobubbles safely ablate vitreous opacities in vivo.Nature nanotechnology · 2022Article
- Article
- A bioinspired gelatin-hyaluronic acid-based hybrid interpenetrating network for the enhancement of retinal ganglion cells replacement therapy.NPJ Regenerative medicine · 2021Article
- Enzymatic vitreolysis using reengineeredMolecular vision · 2021Article
- Importance of the Use of Oxidative Stress Biomarkers and Inflammatory Profile in Aqueous and Vitreous Humor in Diabetic Retinopathy.Antioxidants (Basel, Switzerland) · 2020Review
- Induction of posterior vitreous detachment (PVD) by non-enzymatic reagents targeting vitreous collagen liquefaction as well as vitreoretinal adhesion.Scientific reports · 2020Article
- Transplantation Site Affects the Outcomes of Adipose-Derived Stem Cell-Based Therapy for Retinal Degeneration.Stem cells international · 2020Article
- Vitreous Antioxidants, Degeneration, and Vitreo-Retinopathy: Exploring the Links.Antioxidants (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeVitreous degeneration contributes to several age-related eye diseases, including retinal detachment, macular hole, macular traction syndrome, and nuclear cataracts. Remarkably little is understood about the molecular interactions responsible for maintaining vitreous structure. The purpose of this study was to measure the structural properties of the vitreous body after enzymatic degradation of selected macromolecules.
methodsMechanical properties of plugs of bovine and porcine vitreous were analyzed using a rheometer. Oscillatory and extensional tests measured vitreous stiffness and adhesivity, respectively. Major structural components of the vitreous were degraded by incubation overnight in collagenase, trypsin, or hyaluronidase, singly or in combination. Vitreous bodies were also incubated in hyper- or hypotonic saline. Effects of these treatments on the mechanical properties of the vitreous were measured by rheometry.
resultsEnzymatic digestion of each class of macromolecules decreased the stiffness of bovine vitreous by approximately half (P < 0.05). Differential effects were observed on the damping capacity of the vitreous (P < 0.05), which was shown to correlate with material behavior in extension (P < 0.01). Digestion of hyaluronan significantly decreased the damping capacity of the vitreous and increased adhesivity. Collagen degradation resulted in the opposite effect, whereas digestion of proteins and proteoglycans with trypsin did not alter behavior relative to controls. Osmotic perturbations and double-enzyme treatments further implicated hyaluronan and hyaluronan-associated water as a primary regulator of adhesivity and material behavior in extension.
conclusionsCollagen, hyaluronan, and proteoglycans act synergistically to maintain vitreous stiffness. Hyaluronan is a key mediator of vitreous adhesivity, and mechanical damping is an important factor influencing dynamic vitreous behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.